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| Effect of modified Shaogan Fuzi Decoction on the pathology of joint synovium and expression level of serum IL-2,IL-17A and TNF-α in rats with collagen induced arthritis |
| Hits 1093 Download times 726 Received:September 18, 2020 |
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| DOI
10.11656/j.issn.1673-9043.2021.01.19 |
| Key Words
modified Shaogan Fuzi Decoction;collagen-induced arthritis;inflammatory cytokines |
| Author Name | Affiliation | E-mail | | ZHANG Yuting | Beijing University of Chinese Medicine, Beijing 100029, China | | | HOU Xiujuan | Department of Rheumatology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China | | | LIU Xiaoping | Department of Rheumatology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China | | | ZHU Yuelan | Department of Rheumatology, Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China | zhuyuelanting@163.com |
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| Abstract
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| [Objective] To study the interventional effects of Modified Shaogan Fuzi Decoction on articular synovial pathology and serum IL-2,IL-17A and TNF-α in a rat model of collagen-induced arthritis (CIA),and to explore its mechanism of action.[Methods] Randomly divide 24 rats into normal group,model group,Tripterygium wilfordii group and Shaogan Fuzi Decoction group. Except for the normal group,the other groups used collagen emulsion multi-point intradermal injection to establish an arthritis animal model. After the model was established,gavage was performed separately:the model group was given gavage of 2 mL/(kg·d) of distilled water. The Tripterygium wilfordii group was given gavage of tripterygium wilfordii polyglycoside tablets 6.25 mg/(kg·d),and the Shaogan Fuzi Decoction group was gavaged with Modified Shaogan Fuzi Decoction 12.5 g/(kg·d),and HE staining was used to observe the pathological changes of the ankle joints and synovium in rats. Serum IL-2,IL-17A and TNF-α levels were determined by ELISA.[Results] HE found that Shaogan Fuzi Decoction can improve the inflammation of the joint synovium of the CIA model and inhibit the destruction of articular cartilage.Compared with the normal group,the IL-2 level of the model group increased (P<0.01). Compared with the model group,the IL-2 level of tripterygium wilfordii group and the The Shaogan Fuzi Decoction group decreased (P<0.01);The level of IL-17A in the model group was higher than that in the normal group (P<0.01). Compared with the model group,the IL-17A levels of Tripterygium wilfordii and Shaoganfuzi decoction group decreased (P<0.01);Compared with the normal group,the level of TNF-α in the model group increased (P<0.05). Compared with the model group,TNF-α in the Tripterygium wilfordii group had a downward trend,but there was no statistical difference (P>0.05). The TNF-α level in the Shaogan Fuzi Decoction group TNF-α level decreased (P<0.01),there was a statistical difference between Shaogan Fuzi Decoction group and Tripterygium wilfordii group (P<0.01).[Conclusion] Modified Shaogan Fuzi Decoction can improve the inflammation of the joint synovium of collagen-induced arthritis rats,inhibit the destruction of articular cartilage,and reduce the expression of IL-2,IL-17A and TNF-α in serum. |
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