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| Study on Q-marker of Radix Saposhnikoviae based on UPLC-Q-TOF-MS/MS |
| Hits 1028 Download times 660 Received:September 28, 2020 |
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| DOI
10.11656/j.issn.1673-9043.2021.01.22 |
| Key Words
UPLC-Q-TOF-MS/MS;Radix Saposhnikoviae;Q-marker;chromone;coumarin |
| Author Name | Affiliation | E-mail | | HUO Jinhai | Institute of Chinese Materia Medica, Heilongjiang Academy of Chinese Medicine Sciences, Harbin 150036, China | | | LIU Yajuan | Institute of Chinese Materia Medica, Heilongjiang Academy of Chinese Medicine Sciences, Harbin 150036, China | | | REN Xiaolei | Institute of Chinese Materia Medica, Heilongjiang Academy of Chinese Medicine Sciences, Harbin 150036, China | | | SUN Guodong | Institute of Chinese Materia Medica, Heilongjiang Academy of Chinese Medicine Sciences, Harbin 150036, China | | | LIU Zheng | Institute of Chinese Materia Medica, Heilongjiang Academy of Chinese Medicine Sciences, Harbin 150036, China | | | SUN Jianfeng | Institute of Chinese Materia Medica, Heilongjiang Academy of Chinese Medicine Sciences, Harbin 150036, China | | | WANG Weiming | Institute of Chinese Materia Medica, Heilongjiang Academy of Chinese Medicine Sciences, Harbin 150036, China | zyyjy@163.com |
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| Abstract
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| [Objective] To identify Quality marker in 78 batches of Radix Saposhnikoviae from different origins by ultra performance liquid chromatography coupled with time-of-fight mass spectrometry (UPLC-Q-TOF-MS/MS).[Methods] The UPLC-Q-TOF-MS/MS and electrospray ion (ESI) source were applied for the analysis under the positive ion mode and separation was performed on Waters Acquity UPLC BEH C18 column (2.1 mm×100 mm,1.7 μm),with a mobile phase using water with 0.1% formic acid (A) and acetonitrile with 0.1% formic acid (B) for gradient elution,potential Q-markers were filtered through PCA analysis of Markerview 1.2.1 software,in vivo substance basis research and bioactivity reports from literature. Then the formula of potential Q-markers were determined by accurate mass and isotopic abundance ratio. The structure identification is through the comparison of the reference and the secondary spectrum of the database. Finally,the Q-markers were determined preliminarily by analyzing changes of content between geo-authentic origins and other origins.[Results] Ten differential compounds were identified or inferred between different origins. The geo-authentic origin Q-markers were Ledeouriellol,hamaudol,psoralen and xanthotoxin.[Conclusion] UPLC-Q-TOF-MS/MS method which develops a new strategy can be used for selection of quality evaluation index. It is helpful to make quality evaluation of authentic medicinal materials. |
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