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| Analysis on chemical constituents from extracts of different extraction methods of aerial parts of Glycyrrhiza uralensis by HPLC-MS/MS |
| Hits 1071 Download times 704 Received:January 15, 2021 |
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| DOI
10.11656/j.issn.1673-9043.2021.02.04 |
| Key Words
aerial parts of Glycyrrhiza uralensis;HPLC-MS/MS analysis;flavonoids |
| Author Name | Affiliation | E-mail | | ZHANG Yuehui | School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 102488, China | | | BO Yingyi | Pharmacy Department, Tianjin Baodi Hospital, Tianjin 301800, China | | | WANG Wenquan | School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 102488, China
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China
Engineering Research Center of Good Agricultural Practice for Chinese Crude Drugs, Ministry of Education, Beijing 102488, China | wwq57@126.com | | CHANG Guanhua | School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 102488, China | | | WANG Wendi | School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 102488, China | | | CUI Jie | Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China | | | HOU Junling | School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 102488, China
Engineering Research Center of Good Agricultural Practice for Chinese Crude Drugs, Ministry of Education, Beijing 102488, China | mshjl@126.com | | TAN Weizhong | Sinopharm Group Jinan Co., Ltd., Jinan 250100, China | tanvz@163.com |
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| Abstract
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| [Objective] To analyze chemical constituents from water extract,ethanol extract and CO2 supercritical extract of aerial parts of Glycyrrhiza uralensis.[Methods] HPLC uses Agilent SB-C18 analytical column,acetonitrile-0.5% formic acid solution gradient elution,volume flow rate 1.0 mL/min,column temperature 30℃,injection volum 10 μL under 280 nm detection wavelength. Mass spectrometry uses electrospray ion source,negative ion mode.[Results] In water extract,9 compounds were identified mainly consists of flavonoids (alcohols) glycosides. A total of 14 compounds were identified in ethanol extract,mainly flavonoids (alcohols) aglycones and isopentenyl flavone. In CO2 supercritical extract,10 compounds were identified,mainly isopentenyl flavone.[Conclusion] Extracts of different extraction methods with different properties are acquired providing basis for further research. |
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