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| Effect of Zizhu Ointment on inflammatory response and NF-kB/JNK/PI3K signal expression of diabetic foot ulcer infected by staphylococcus aureus |
| Hits 950 Download times 685 Received:November 25, 2020 |
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| DOI
10.11656/j.issn.1673-9043.2021.02.18 |
| Key Words
Zizhu Ointment;diabetic foot ulcer;staphylococcus aureus;inflammation;mercury;NF-κB |
| Author Name | Affiliation | E-mail | | HAN Qiang | Department of Peripheral Vascular Diseases, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
Beicai Health Service Center, Pudong New Area, Shanghai 200000, China | | | LIU Guobin | Department of Peripheral Vascular Diseases, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China | drliuguobin@163.com | | QIN Liang | Medical Instrumentation College, Shanghai University of Medicine&Health Sciences, Shanghai 200000, China | | | YAN Shimeng | Department of Peripheral Vascular Diseases, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China | | | HUANG Renyan | Department of Peripheral Vascular Diseases, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China | | | XU Feng | Department of Peripheral Vascular Diseases, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China | | | SHI Chenyan | Department of Peripheral Vascular Diseases, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China | | | CHEN Junhao | Department of Peripheral Vascular Diseases, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China | |
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| Abstract
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| [Objective] Diabetic foot ulcer is a serious lower extremity complication of diabetes. The long-term treatment of diabetic foot ulcer is prone to secondary bacterial infection. Infection has a significant delay effect on the healing of ulcer. Staphylococcus aureus is a common infection pathogen of diabetic foot ulcers. Persistent inflammation caused by infection is an important factor that leads to the continuation of diabetic foot ulcers,which is also a clinical problem in the treatment of diabetic foot ulcers. Zizhu Ointment is an effective prescription for the treatment of diabetic foot ulcers in our hospital. This study aims to explore the mechanism of the effect of traditional Chinese medicine Zizhu ointment on the healing of diabetic foot ulcer model infected with Staphylococcus aureus from the perspective of infectious inflammation.[Methods] Using high fat feed composition chain urea with cephalosporins (STZ) caused by diabetes,constant temperature constant pressure burns instrument experimental diabetic foot ulcer model was established,through the subcutaneous standard strains caused by infection,made from experimental models,ordinary ulcer model in comparison with the experimental model with infective diabetic foot ulcer inflammatory response characteristics,through the contrast of interference from the experimental models including normal saline,Western medicine Bei Fuji,different concentration gradient of Zizhu Ointment intervention for 14 days,using ELISA method to detect serum nitric oxide (NO),C-reactive protein (CRP) and interleukin 6 (IL-6),Western blot (WB) to detect nuclear factor-kappa Bp65 predominate (p65 nf-kappa B),c-Jun-N-terminal kinase (JNK) and phosphatidyl inositol 3 kinase (PI3K) path way signal molecules,immunohistochemical method to detect wound tissue of vascular endothelial growth factor (VEGF) and platelet endothelial cell adhesion molecule,34 (CD34),cold atomic absorption method to detect the contents of blood mercury (Hg),study the Zizhu Ointment to staphylococcus aureus infection of diabetic foot ulcer inflammation effect and drug safety.[Results] The expression of inflammatory factors in the diabetic foot ulcer model with Staphylococcus aureus infection was higher than that in the normal ulcer model,and Zizhu Ointment could reduce the expression of inflammatory factors (NO,CRP and IL-6) in the diabetic foot ulcer model. Infective diabetic foot ulcer model in the nf-kappa B,JNK,PI3Ks inflammatory signaling pathways activated,Zizhu Ointment can obviously inhibit the JNK and nf-kappa B P65,JNK,phosphorylation of PI3K,reduce wound inflammation,at the same time increase the wound the expression of VEGF,promote angiogenesis,as well as the Zizhu Ointment medication rats blood mercury levels of testing instructions medication has good security.[Conclusion] The diabetic foot ulcer model of rats infected with Staphylococcus aureus has a higher level of inflammation than ordinary ulcer models;Zizhu ointment can reduce the inflammation level of diabetic foot ulcers infected by Staphylococcus aureus and inhibit the inflammation inflammation-related signaling pathway activation,increased angiogenesis factors CD34 and VEGF stimulate wound angiogenesis to promote tissue repair and improve wound healing,showing that Zizhu ointment is a safe and effective drug for treating diabetic foot ulcers and has good application prospects. |
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