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Effect of Shuimu Hening Formulation on autophagy function of rotenone-induced Parkinson's model mice
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DOI   10.11656/j.issn.1673-9043.2021.02.19
Key Words   Shuimu Hening Formulation;Parkinson's disease;rotenone;autophagy;mechanism of action
Author NameAffiliationE-mail
LIU Ping Shandong Maternal and Child Health Care Hospital, Department of Traditional Chinese Medicine, Jinan 250014, China  
XU Longjin Shandong Provincial Center for Disease Control and Prevention, Jinan 250014, China  
HUO Qing The Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Neurosurgery Department, Jinan 250011, China hq3701@163.com 
QIU Zhaoyang Weifang Medical College, Weifang 261000, China  
Abstract
    [Objective] To observe the intervention effects of Shuimu Hening Formulation on the behavior of Parkinson disease(PD) model mice and TH,alpha-syn and autophagy related proteins in the substantia nigra of the midbrain.[Methods] PD mouse model was prepared by subcutaneous injection of rotenone into the neck and back.The mice were randomly divided into high-dose group,medium-dose group,low-dose group,medoba group and model group. Shuimu Hening Formulation were given to the high,medium and low dose groups,medoba was given to themedoba group,and distilled water was given to the model group and normal group.The above groups were given for 28 consecutive days. The behavior of mice was observed. TH,α-syn and autophagy related proteins LC3,p62 and Beclin1 were determined by immunohistochemistry,western blot and RT-PCR.[Results] 1)After the model building,the behavior of mice was changed,which was mainly manifested as slow movement,slow reaction,weakened anti-arrest reaction,rough and disorderly hair,and yellow color. After the treatment of Shuimu Hening Formulation andmedoba,the above symptoms were improved. 2) Both Shuimu Hening Formulation and medoba can increase the expression of TH in midbrain tissue of PD model mice and reduce the expression of alpha-syn. Both of them can achieve the therapeutic effect by intervening autophagy related proteins. 3)The effect of the medium dose groupwas better than that of other groups.[Conclusion] These results suggest that Shuimu Hening Formulation can up-regulate the autophagy level,improve the autophagy obstacle,promote the smooth completion of autophagy flow,and degrade the abnormal aggregation of alpha-syn,thus protecting DA neurons.

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