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| Protective effect and mechanism of Xinnaoning Capsule on cerebral ischemia-reperfusion injury by regulating NF-κB mediated anti-inflammatory pathway |
| Hits 918 Download times 598 Received:January 27, 2021 |
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| DOI
10.11656/j.issn.1673-9043.2021.03.19 |
| Key Words
cerebral ischemia reperfusion;Xinnaoning Capsule;NF-κB |
| Author Name | Affiliation | E-mail | | REN Jie | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Research and Development Center of Traditional Chinese Medicine, Tianjin International Joint Academy of Biotechnology & Medicine, Tianjin 300457, China | | | WANG Yule | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Research and Development Center of Traditional Chinese Medicine, Tianjin International Joint Academy of Biotechnology & Medicine, Tianjin 300457, China | | | HE Shuang | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Research and Development Center of Traditional Chinese Medicine, Tianjin International Joint Academy of Biotechnology & Medicine, Tianjin 300457, China | | | LI Zhixiong | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Research and Development Center of Traditional Chinese Medicine, Tianjin International Joint Academy of Biotechnology & Medicine, Tianjin 300457, China | | | ZHU Yan | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Research and Development Center of Traditional Chinese Medicine, Tianjin International Joint Academy of Biotechnology & Medicine, Tianjin 300457, China | yanzhu.harvard@icloud.com |
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| Abstract
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| [Objective] To observe the effect of Xinnaoning Capsule on cerebral ischemia reperfusion injury.[Methods] Male ICR mice were randomly divided into sham-operation group,model group,Xinnaoning group and Edaravone group. After the pre-dosed 7 days,a mouse middle artery occlusion (MCAO) model was established,and ischemia for 30 minutes,reperfusion for 24 h. The evaluation of nerve function damage,tissue edema,infarct area and histopathological damage were carried out in sequence;the core target of Xinnaoning Capsule's active components on cerebral ischemia reperfusion injury was analyzed by network pharmacology;Western blot and immunohistochemistry were used to determine the expression of nuclear transcription factor-B (NF-κB p65).[Results] The neurobehavioral score,brain midline dislocation distance,and infarct area of the Xinnaoning group were significantly lower than those of the model group (P<0.01);the results of hematoxylin-eosin (HE) staining showed that compared with the model group,the Xinnaoning group was improved in terms of pathological damage;network pharmacological analysis shows that the mechanism of Xinnaoning capsules is closely related to RelA (p65);Western blot results show that the expression of total NF-κB p65 protein in Xinnaoning group was significantly lower than that of model group (P<0.01);the results of immunohistochemical staining showed that the number of positive cells in the Xinnaoning group was significantly less than that of the model group (P<0.01). the difference with the model group was more significant if the integral optical density measurement method was used,(P<0.001).[Conclusion] Xinnaoning Capsule effectively improved cerebral ischemia-reperfusion injury in MCAO mice via at least in part by an anti-inflammatory mechanism indicated by reduction of the expression and activation of NF-κB. |
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