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| Effect of betulin on the expression of APH-1 and PEN-2 in hippocampal neurons of Alzheimer's model rats |
| Hits 967 Download times 601 Received:January 22, 2021 |
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| DOI
10.11656/j.issn.1673-9043.2021.03.22 |
| Key Words
betulin;senile dementia;SD rat;APH-1;PEN-2;γ-secretase |
| Author Name | Affiliation | E-mail | | JIN Yan | Department of Neurology, The Third Affiliated Hospital of Qiqihar Medical University, Qiqihar 161002, China | | | FU Zenghui | Department of Neurology, The Third Affiliated Hospital of Qiqihar Medical University, Qiqihar 161002, China | fzh92@foxmail.com | | LIN Zaihong | Department of Neurology, The Third Affiliated Hospital of Qiqihar Medical University, Qiqihar 161002, China | | | JIANG Yan | Department of Neurology, The Third Affiliated Hospital of Qiqihar Medical University, Qiqihar 161002, China | | | LIU Jing | Department of Neurology, The Third Affiliated Hospital of Qiqihar Medical University, Qiqihar 161002, China | | | DU Shu | Department of Neurology, The Third Affiliated Hospital of Qiqihar Medical University, Qiqihar 161002, China | | | ZHANG Guangping | Department of Neurology, The Third Affiliated Hospital of Qiqihar Medical University, Qiqihar 161002, China | | | CHEN Tuantuan | Department of Neurology, The Third Affiliated Hospital of Qiqihar Medical University, Qiqihar 161002, China | |
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| Abstract
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| [Objective] To investigate the intervening effect and possible mechanism of betulin in Alzheimer's disease rats,so as to provide new possibilities for exploring the treatment of senile dementia.[Methods] Healthy adult SD rats were randomly divided into control group,model group,low-dose group and high-dose group. The 12 rats in each group were administrated by gavage continuously for 3 months,and the relevant indexes were detected by Morris water maze experiment. The activity of γ-secretase was detected by double luciferase reporter gene system,the expression levels of APH-1,HIF-1,PEN-2 and CREB were detected by real-time PCR and western blotting.[Results] Water maze test showed that betulin ginseng can significantly improve the cognitive dysfunction in model rats;dual luciferase reporter system detection results showed that betulin can inhibit the gamma secretase activity;the detection result of real-time PCR and western blotting showed that betulin can inhibit PEN-2 and CREB expression,but no change in the expression of APH-1 and HIF-1.[Conclusion] Betulin can significantly improve AD model rats's behavior of learning,memory ability and spatial cognitive ability. Betulin can inhibit the hippocampal CA1 region of PEN-2 related genes and proteins,but the APH-1 related gene and protein have no significant influence. |
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