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| Effect and mechanism of Kangxian Pills againstchronic liver injury in mice by activating Nrf2 signaling pathway |
| Hits 952 Download times 541 Received:January 28, 2021 |
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| DOI
10.11656/j.issn.1673-9043.2021.04.19 |
| Key Words
Kangxian Pills;chronic liver injury;oxidative stress;Nrf2 |
| Author Name | Affiliation | E-mail | | CAO Min | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | WANG Li | Tianjin Second People's Hospital, Tianjin 300192, China | | | LIU Haizhao | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | PAN Zanhong | Tianjin Second People's Hospital, Tianjin 300192, China | | | LIU Jianwei | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | 13389915877@126.com | | BIAN Yuhong | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | bianyuhong_2012@163.com |
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| Abstract
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| [Objective] To investigate the protective effect and mechanism of Kangxian Pills on CCl4 induced chronic liver injury in mice. [Methods] BALB/c mice were divided into normal group, model group, diammonium glycyrrhizinate group[60 mg/(kg·d)] and Kangxian pills group[3 g/(kg·d)]. CCl4 was injected intraperitoneally to establish the model of chronic liver injury in mice, at the same time, the same volume of olive oil was injected intraperitoneally in the normal group. After the model was established successfully, the medicine were given for 4 weeks. We detected the serum AST and ALT levels, HE staining was used to observe the pathological changes of liver tissue, and the collagen deposition in liver tissue was observed by Masson staining; the expression of α-SMA in liver tissue was observed by immunohistochemistry; the content of SOD, GSH-Px and MDA in liver tissue were detected, mRNA and protein expression of Nrf2, HO-1, Gclc and NQO1 in liver tissue were detected by qPCR and Western Blot. [Results] Compared with the normal group, the levels of serum AST and ALT in the model group were significantly increased; the expression of collagen, α-SMA and MDA in liver tissues were significantly increased while the activities of SOD and GSH-Px were significantly decreased. In addition, the mRNA expression levels of Nrf2, HO-1, Gclc and NQO1 and the protein expression levels of Nrf2, Gclc and NQO1 in liver tissues were reduced to different degrees. Compare with the mice with chronic liver disease, Kangxian Pills group could significantly decreased the activities of AST and ALT in serum, reduced the content of collagen in liver tissue and inhibitedthe expression of α-SMA; reduced the content of MDA in liver and increased the content of SOD and GSH-Px in liver. In addition, the mRNA and protein expression of Nrf2, HO-1, Gclc and NQO1 in liver were also significantly up-regulated. [Conclusion] Kangxian Pills could protect CCl4induced chronic liver injury in mice, and its mechanism may be to activate of Nrf2 signaling pathway and inhibition of oxidative stress in hepatocytes. |
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