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| Effect of Taohong Siwu Decoction combined with metformin on fracture healing in rats model of type 2 diabetes mellitus complicated with osteoporosis |
| Hits 1015 Download times 637 Received:June 22, 2021 |
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| DOI
10.11656/j.issn.1673-9043.2021.05.18 |
| Key Words
Taohong Siwu Decoction;metformin;osteoporosis;fracture;type 2 diabetes mellitus |
| Author Name | Affiliation | | LI Lei | Joint Trauma Surgery Department, Bazhou People's Hospital, Korla 841000, China | | CHEN Wanhai | Joint Trauma Surgery Department, Bazhou People's Hospital, Korla 841000, China | | Narencaoke | Medical Records Management Information Department, Bazhou People's Hospital, Korla 841000, China |
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| Abstract
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| [Objective] To investigate the effect of Taohong Siwu Decoction combined with metformin on fracture healing in rats model of type 2 diabetes mellitus (T2DM) complicated with osteoporosis (OP).[Methods] The 40 SPF female rats were randomly divided into normal group, control group, Taohong Siwu Decoction group and combination group, with 10 rats in each group. Model of T2DM complicated with OP fracture were established in all rats except for the normal group, the fracture model were established in all four groups. The normal group and the control group were given 10 ml/kg of normal saline everyday, the Taohong Siwu Decoction group was given 6 g/kg of Taohong Siwu Decoction everyday, and the combination group was given 100 mg/kg of metformin saline suspension on this basis. After the treatments, fracture healing states of all rats were inspected by X-ray;bone mineral density (BMD) of each rat was detected by dual energy X-ray absorptiometry (DEXA);bone histomorphologies were observed by HE staining;bone volume (BV), trabecular number (Tb.N), trabecular thickness (Tb.Th), trabecular resolution (Tb.Sp), junction density (Conn.D), bone volume fraction (BV/TV) and bone surface volume ratio (BS/BV) were measured by microCT;biomechanical properties of femoral were detected by mechanical tester;fasting blood glucose (FBG) of each rat was detected at the same time of every week during the experiment;serum vascular endothelial growth factor (VEGF), fibrinogen (FIB), alkaline phosphatase (ALP) and D-dimer were detected.[Results] The X-ray showed that the fracture lines of Taohong Siwu Decoction group and combination group wereblurry, bone callus connected to fracture end. As compared with control group, BMD of Taohong Siwu Decoction group and combination group were significantly increased (P<0.05), and combination group was higher than Taohong Siwu Decoction group (P<0.05). HE staining showed that osseous callus of Taohong Siwu Decoction group and combination group were increased, braided bone and lamellar bone began to fuse. Tb.N, Tb.Th, Conn.D and BV/TV of Taohong Siwu Decoction group and combination group were higher than control group (P<0.05), Tb.Sp and BS/BV were lower than control group (P<0.05);as compared with Taohong Siwu Decoction group, Tb.N, Tb.Th, Conn.D and BV/TV of combination group were higher and BS/BV was lower (P<0.05). Max loads of Taohong Siwu Decoction group and combination group were greater than control group (P<0.05);and max load of combination group was greater than Taohong Siwu Decoction group (P<0.05). FBG of Taohong Siwu Decoction group and combination group were decreased to close to normal group after the treatment. Compared with the control group, the expression levels of VEGF, FIB, ALP and D-dimer in the Taohong Siwu Decoction group and the combination group were significantly reduced(P<0.05). Compared with the Taohong Siwu Decoction group, the expression levels of VEGF, FIB, ALP and D-dimer in the combination group were significantly reduced (P<0.05). The expression level of FIB and D-dimer were lower, and the expression level of ALP were higher (P<0.05).[Conclusion] Taohong Siwu Decoction combined with metformin could effectively decrease the blood glucose level, increase the BMD and biomechanical property osteoporosis rats, improve the formation of callus, decrease the expression levels of serum VEGF, FIB, ALP and D-dimer, which promotes the fracture healing ofrats model of T2DM complicated with OP. |
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