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| Effect of Ershiwuwei Feibing Pills on microvessel dysfunction and underlying mechanism in zebrafish |
| Hits 779 Download times 816 Received:August 10, 2021 |
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| DOI
10.11656/j.issn.1673-9043.2021.06.18 |
| Key Words
Ershiwuwei Feibing Pills;zebrafish;microvessel dysfunction |
| Author Name | Affiliation | E-mail | | QIANG Ba | Tibet Qizheng Tibetan Medicine Co., Ltd., Lasa 850030, China | | | GUO Shengya | Hunter Biotechnology Inc., Hangzhou 310051, China | | | CHEN Lunju | Tibet Qizheng Tibetan Medicine Co., Ltd., Lasa 850030, China | | | XU Yiqiao | Hunter Biotechnology Inc., Hangzhou 310051, China | | | LI Feng | Tibet Qizheng Tibetan Medicine Co., Ltd., Lasa 850030, China | lfz1901@qzh.cn |
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| Abstract
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| [Objective] To investigate whether Ershiwuwei Feibing Pills (EFP) could mitigate microvessel dysfunction and the underlying mechanism.[Methods] Zebrafish was treated with arachidonic acid to induce models of thrombosis and zebrafish heart output and blood flow velocity were detected in zebrafish models with or without treatment of EFP. Simvastatin was used to induce microvascular defect and the vascular area of the zebrafish intestine and the intervascular diameter were calculated. Similarly,ponatinib was adopted to cause vascular endothelial injury and the thrombus in the tail vein was observed with microscope.[Results] Compared with the control group,the EFP group (27.8 mg/L) group had a significant increase in cardiac output (0.11 nL/s vs 0.19 nL/s, P<0.05),and a significant increase in blood flow velocity (398 μm/s vs 693 μm/s,P<0.001). The area of intestinal blood vessels was increased (19 335 pixels vs 25 041 pixels,P<0.05),and the promotion of microvascular regeneration was enhanced. Furthermore,after treatment with EFP (83 and 250 μg/mL),the diameter of zebrafish internode vessels increased significantly (8.7 vs 11.5 vs 11.8 pixels,P<0.001),and the incidence of thrombosis decreased significantly.[Conclusion] EFP can improve microvessel dysfunction,which may result from improving microvascular hemodynamics,reducing vascular endothelial damage and promoting endothelial regeneration and thrombosis. |
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