|
| Network pharmacology-based molecular mechanisms of Hedan Tablets for hyperlipidemia regression |
| Hits 903 Download times 741 Received:August 10, 2021 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1673-9043.2021.06.22 |
| Key Words
Hedan Tablets;hyperlipidemia;network pharmacology;disease-tarqet network;mechanism |
| Author Name | Affiliation | E-mail | | YAO Minghe | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | YAN Haifeng | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China | | | WANG Jing | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China | | | XU Zongpei | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LI Yuhong | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | liyuhong@tjutcm.edu.cn |
|
| Abstract
|
| [Objective] The objective of the present study was to investigate the possible underlying cellular mechanisms responsible for Hedan Tablets' effects on hyperlipidemia based on network pharmacology.[Methods] Network pharmacology method was utilized to disclose the association between active component-target-hyperlipidemia and the molecular mechanism of Hedan Tablets on the treatment of hyperlipidemia. Chemical active components and the targets of the 5 herbs in Hedan Tablets were retrieved from TCMSP and ETCM databases. UniProt and DisGeNET databases were used to obtain the corresponding genes of the targets and the disease targets of hyperlipidemia,respectively. String data platform and Cytoscape was used to visualize and construct the network-based diagram for active component interaction target,protein-protein interaction network(PPI),and active component-disease target. Finally,DAVID bioinformatics tools were used to analyze the go-function and KEGG pathway enrichment of related targets.[Results] An active component-target-hyperlipidemia and network pathway revealed that 61 active compounds deduced from Hedan Tablets regulated 231 close targets common to Hedan Tablets and hyperlipidemia. Analysis of the common targets indicated that the anti-hyperlipidemia effects of Hedan Tablets might be related to 20 potential targets in hyperlipidemia. Visualized network diagram disclosed ergosterol,guercetol, corylinal,etc. as critical active-compounds and PTGS1,ADRB2,F2,PPARG,etc. as crucial targets. Also,the go-enrichment analysis revealed that the plausible mechanism of Hedan Tablets anti-hyperlipidemia action might be through the regulation of oxidoreductase activity,lipid and ion binding,signal transduction activity,nucleic acid binding transcription factor activity,lipid metabolism,single organism metabolism,small molecule metabolism,cell proliferation, and other life processes. KEGG pathway enrichment analysis showed that Hedan tablet played a pharmacological role in hyperlipidemia by regulating serotonin containing synapses,PPAR signaling pathway,drug metabolism cytochrome P450,arachidonic acid metabolism,linoleic acid metabolism,and other pathways.[Conclusion] Altogether,the study reveals the possible cellular processes likely to be influenced by Hedan Tablets against hyperlipidemia,and provides the platform for further pharmacological evaluation in vivo models. |
|
|
|
|
|
