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| The study on mechanism of the anti-tumor effect of Fufang Huangqi Oral Solution combined chemotherapy |
| Hits 651 Download times 527 Received:October 18, 2021 |
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| DOI
10.11656/j.issn.1673-9043.2022.01.18 |
| Key Words
Fufang Huangqi Oral Solution;H22 tumor-bearing mice;Th1 cell;Treg cell |
| Author Name | Affiliation | E-mail | | GU Yingyuan | Changzhou Hospital of Traditional Chinese Medicine, Changzhou 213003, China | | | PU Xiaojiao | Yunnan University of Traditional Chinese Medicine, Kunming 650500, China | | | YIN Na | Yunnan University of Traditional Chinese Medicine, Kunming 650500, China | | | WAN Chunping | The NO.1 Affiliated Hospital of Yunnan University of Traditional Chinese Medicine, Kunming 650021, China | wanchunping1012@163.com |
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| Abstract
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| [Objective] This paper was designed to investigatethe anti-tumor effect of Fufang Huangqi Oral Solution (FFHQKY) combined chemotherapyin H22 tumor-bearing mice and reveal its mechanism. This study will provided a basis for the theoretical foundation of antitumor immunotherapy of FFHQKY.[Methods] The H22 tumor-bearing mice were established to investigate the effect ofanti-tumor immunological effect of FFHQKY combined chemotherapy. Mice were divided into four groups, including model group, FFHQKY treated-group, cyclophosphamide(CTX) group, and combination treated group. After 16 days of intragastric administration, the tumor tissue were isolated to assess the anti-tumor effect of FFHQKY, the lymphocyte cells proliferation was detected by MTT method, the total cell numbers of leukocyte, monocyte, neutrophil were measured by hematology analyzer. The expression of Treg (CD4+Foxp3+) and Th1 cells(CD4+IFN-γ+) were detected by flow cytometry.[Results] Compared with CTX group, the combination treated group had no influence on inhibitory rate of tumor but significantly recovered the lymphocyte proliferation activity from H22 tumor-bearing mice in response to ConA, increased the cells count of leukocyte and neutrophil. Importantly, compared with CTX group, the percent of Th1 cellsTh1 cells (CD4+IFN-γ+) were increased in combination treated group. Furthermore, combination treatment reduced the expression of Treg (CD4+Foxp3+).[Conclusion] Combination FFHQKY with chemotherapy exerted significantly anti-tumor immunological, the mechanism of action may be linked to activate Th1 cells response and down-regulated Treg expression, consequently enhance anti-tumor immunological effect. |
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