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| Experimental study of Yishen Huashi Granule inproving early renal tubular injury in diabetic mice |
| Hits 683 Download times 440 Received:December 25, 2021 |
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| DOI
10.11656/j.issn.1673-9043.2022.02.19 |
| Key Words
Yishen Huashi Granule;db/db mice;megalin;cubilin;NGAL;early renal tubular injury |
| Author Name | Affiliation | E-mail | | CHEN Li | Beijing University of Chinese Medicine, Beijing 100029, China | | | AN Haiyan | Dongfang Hospital Affiliated to Beijing University of Chinese Medicine, Beijing 100078, China | | | GUO Xiaoyuan | Dongfang Hospital Affiliated to Beijing University of Chinese Medicine, Beijing 100078, China | | | ZHANG Chengcheng | Dongfang Hospital Affiliated to Beijing University of Chinese Medicine, Beijing 100078, China | zhangcheng2135@163.com |
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| Abstract
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| [Objective] To investigate the effect of Yishen Huashi Granule on early renal tubular injury in spontaneous type 2 diabetic db/db mice. [Methods] The 18 SPF male spontaneous type 2 diabetic db/db mice aged 6~7 weeks were randomly divided into model group,valsartan group[13.33 mg/(kg·d)] and Yishen Huashi group[5 g/(kg·d)],with 6 mice in each group. Another 6 male non-diabetic db/m mice were set as normal group. The normal group and model group were given 10 mL/(kg·d) deionized water by gavage for 12 weeks. 24 h urine was collected by metabolic cage for 24 h urine protein (24 h-UTP) and neutrophil-gelatinase-associated lipid-carrying proteins (NGAL) detection. After 12 weeks,the mice were anesthetized and blood samples were taken. The levels of Scr,BUN,UA,TG and LDL were detected by automatic biochemical analyzer. The renal pathological morphology changes were observed by light microscope with PAS staining. The expression intensity of megalin and cubilin in renal tissues were detected by immunohistochemistry. [Results] After the intervention of Yishen Huashi Granule,the levels of UA and LDL in blood,24 h-UTP and NGAL in urine of db/db mice were decreased(P<0.05,P<0.01) compared with the model group. PAS staining showed that mesangial matrix proliferation and tubule injury were alleviated,and the protein expression of megalin and cubilin in renal tubular epithelial cells were increased. According to the statistics of Imagepro Plus 6.0 software,the glomerular mesangial matrix index decreased (P<0.01),and the mean optical density of megalin and cubilin increased (P<0.05). [Conclusion] Yishen Huashi Granule can reduce the formation of urine protein by increasing the expression of megalin and cubilin protein on the kidney tubes of db/db mice,and play a role in protecting the renal tube. |
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