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| Anti-tumor effect study of Astragalus polysaccharide promotes dendritic cell through TLR4 signal pathway |
| Hits 674 Download times 510 Received:December 20, 2021 |
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| DOI
10.11656/j.issn.1673-9043.2022.02.20 |
| Key Words
Astragalus polysaccharides;dendritic cell;TLR4 pathway;anti-tumor |
| Author Name | Affiliation | E-mail | | JIA Beitian | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | WANG Li | Tianjin Second People's Hospital, Tianjin 300192, China | | | CUI Huantian | Shandong University, Qingdao 266237, China | | | JIN Yutong | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | CAO Min | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LIU Haizhao | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | BIAN Yuhong | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | bianyuhong_2012@163.com |
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| Abstract
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| [Objective] The aim of this study is to investigate the anti-tumor effect of Astragalus polysaccharides (APS) activated dendritic cell (DCs) via the TLR4 signaling pathway. [Methods] The DC2.4 were randomly divided into Control group,APS group,TNF-α group,APS+TNF-α group,TAK-242+TNF-α group,TAK-242+APS+TNF-α group,ST2825+TNF-α group and ST2825+APS+TNF-α group. After 72h of intervention,the ratio of CD80+CD86+ DCs were detected by flow cytometry,and the effect of DCs on the secretion of cytokine IL-12 were detected by ELISA. Then the DCs in previous step were co-cultured with T cells,and effects of DCs on T cell activation were detected by ELISA;the activated CTL cells were co-cultured with tumor cells CT 26,tumor-cell-killing efficiency was calculated using the full-field cell imaging analyzer. [Results] Compared with the control group,APS can significantly promote the expression of CD80+CD86+ DCs and the secretion of IL-12 (P<0.05),and the best effects were obtained when APS and TNF-α were synergistically intervened. In addition,the DCs intervened by APS+TNF-α could significantly promote proliferation of T cells,the secretion of IL-2 by T cells and enhance the killing of tumors by CTL (P<0.05). However,after adding the TLR4 pathway blocker TAK-242 or ST2825,the expression of CD80+CD86+ DCs and the secretion level of IL-12 in APS+TNF-α group were not significantly different from those in the TNF-α group. Compared with the TNF-α group,there was no significant difference in proliferation of T cells,the level of IL-2 secretion and tumor killing by T cells in APS+TNF-α group with blocker TAK-242 or ST2825. [Conclusion] APS could induce DCs maturation and increase the activating ability of DC on T cells. These effects and mechanism may through TLR4/MyD88 pathway,and APS/TNF-a combination treatment to DCs is the best. |
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