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| Simultaneous determination of multicomponent in classical herbal prescription Jichuan Decoction by HPLC method |
| Hits 596 Download times 384 Received:December 24, 2021 |
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| DOI
10.11656/j.issn.1673-9043.2022.02.22 |
| Key Words
classical famous prescription;Jichuanjan Decoction;multicomponent;determination of contents;HPLC |
| Author Name | Affiliation | E-mail | | CAI Nan | School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Traditional Chinese Medicine Research Center, Tasly Holding Group Co., Ltd., Tianjin 300410, China | | | CAO Ningning | School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | ZHANG Wanjing | School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | PEI Shuai | School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LI Xiaoxuan | School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | XIAO Xuefeng | School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | kai1219@163.com | | LIU Changxiao | State Key Laboratory of Drug Delivery Technology and Pharmacokinetics, Tianjin Institute of Pharmaceutical Research, Tianjin 300193, China | liuchangxiao@163.com |
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| Abstract
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| [Objective] To establish an HPLC method for simultaneous determination of echinacoside,verbascoside,ligustilide,naringin,neohesperidin and isoferulic acid in Jichuan Decoction,and provide a scientific basis for its quality control. [Methods] The HPLC separation was performed on Waters WAT054275 C18 chromatographic column (4.6 mm×250 mm,5 μm),with gradient elution using the mobile phase consisting of 0.1% phosphoric acid solution and acetonitrile at the flow rate of 1.0 mL/min. The column temperature was 30℃,detection wave-length was 310 nm and the injection volume was 10 μL. [Results] The calibration curve was liner within the range of 5.18~51.84 μg/mL (r=0.999 6) for echinacoside,3.60~36.00 μg/mL(r=0.999 4) for verbascoside,11.22~112.20 μg/mL(r=0.999 6) for ligustilide,20.80~208.00 μg/mL(r=0.999 1) for naringin,14.80~148.00 μg/mL (r=0.999 9) for neohesperidin,0.98~9.80 μg/mL(r=0.999 3) for isoferulic acid,and the average recovery were 100%,99.2%,99.0%,99.5%,99.4%, and 98.1%;with RSD were 0.95%,0.36%,0.56%,0.92%,1.11%,and 3.42%. [Conclusion] The method is stable,highly specific,accurate,reproducible,and can provide reference for the quality evaluation of classical famous prescription Jichuan Decoction. |
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