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| Mechanism of Erhuang Yishen Decoction in treating chronic renal failure via inactivation of the TGF-β1/Smad signal pathway |
| Hits 644 Download times 340 Received:February 22, 2022 |
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| DOI
10.11656/j.issn.1673-9043.2022.03.14 |
| Key Words
renal failure;serum creatinine;transforming growth factor-β1;renal interstitium |
| Author Name | Affiliation | E-mail | | DONG Bin | Jining Hospital of Traditional Chinese Medicine, Jining 272004, China | | | YU Bin | College of Integrative Medicine, Jining Medical College, Jining 272067, China | | | SUN Min | College of Integrative Medicine, Jining Medical College, Jining 272067, China | | | LIU Zhihua | Jining Hospital of Traditional Chinese Medicine, Jining 272004, China | liuzhihua98003@163.com | | WANG Lerong | Jining Hospital of Traditional Chinese Medicine, Jining 272004, China | | | WANG Xiangsheng | Jining Hospital of Traditional Chinese Medicine, Jining 272004, China | |
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| Abstract
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| [Objective] To observe the effect of Erhuang Yishen Decoction(EHYST) on serum creatinine (BUN), serum creatinine (Scr), transforming growth factor-β1 (TGF-β1), Smad3, Smad4, Smad7 mRNA and protein expression levels in chronic renal failure via inactivation of the TGF-β1/Smad signal pathway.[Methods] Healthy SPF male SD rats were used to establish the animal model of chronic renal failure by 5/6 nephrectomy. After successful modeling, they were randomly divided into model group, Niaoduqing group(NDQ) and EHYST group. The drug groups were given the corresponding drugs for gavage treatment for 12 weeks. At the end of the experiment, BUN and Scr contents were measured, and pathological changes were analyzed by HE staining. TGF-β1, Smad3, Smad4 and Smad7 mRNA and protein expression were detected by RT-PCR and western blot.[Results] The animal model of chronic renal failure could be successfully established by 5/6 nephrectomy. Compared with the model group, the EHYST group and the NDQ group could reduce the Scr and BUN contents, and the difference was statistically significant(P<0.01). In the EHYST Decoction group and NDQ group, a small amount of inflammatory cells infiltrated and fibrous hyperplasia were observed in the renal interstitium, and the area of fibrosis was alleviated, which could improve the degree of renal fibrosis in renal failure. The results of gene and protein expression analysis showed that compared with the model group, the expression levels of TGF-β1, Smad3, and Smad4 in the EHYST Decoction group and NDQ group showed a decreasing trend, and the difference was statistically significant(P<0.05), and the expression levels of Smad7 showed an increasing trend, and the difference was also statistically significant(P<0.05).[Conclusion] EHYST could improve the degree of renal fibrosis, inhibit the gene and protein expression of TGF-β1, Smad3 and Smad4, and promote the increased expression of Smad7 in rats with chronic renal failure, which may play a related therapeutic role through regulating TGF-β1/Smad signaling pathway. |
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