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| Effect of Semen Lepidii aqueous extract on neonatal rats with LPS inhalation lung injury by regulating HMGB1/TLR4/NF-κB signaling pathway |
| Hits 674 Download times 393 Received:March 11, 2022 |
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| DOI
10.11656/j.issn.1673-9043.2022.03.15 |
| Key Words
lung injury;Semen Lepidii;inflammatory reaction;HMGB1/TLR4/NF-κB signaling pathway |
| Author Name | Affiliation | | FENG Lingling | Department of Pediatrics, Xiantao First People's Hospital Affiliated to Yangtze University, Xiantao 433000, China | | DU Xin | Department of Pediatrics, Xiantao First People's Hospital Affiliated to Yangtze University, Xiantao 433000, China | | GE Yu | Department of Pediatrics, Xiantao First People's Hospital Affiliated to Yangtze University, Xiantao 433000, China | | YANG Fan | Department of Pediatrics, Xiantao First People's Hospital Affiliated to Yangtze University, Xiantao 433000, China | | HUANG Rong | Department of Pediatrics, Xiantao First People's Hospital Affiliated to Yangtze University, Xiantao 433000, China | | ZHANG Liping | Department of Pediatrics, Affiliated Hospital of Wuhan University of Science and Technology, Wuhan 430000, China |
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| Abstract
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| [Objective] To investigate the effect of Semen Lepidii aqueous extract on neonatal rats with LPS aspiration pneumonia.[Methods] Rat models of aspiration lung injury were prepared by intraperitoneal injection of liposaccharide(LPS). The rats were randomly divided into 6 groups:control group, model group, water extract of Semen Lepidii(5 g/kg), water extract of Semen Lepidii (10 g/kg), water extract of Semen Lepidii (20 g/kg), and dexamethasone (0.002 g/kg). The ratio of dry and wet lung tissue was measured by dry and wet method. HE staining was used to detect pathological damage of lung tissue. The levels of superoxide dismutase(SOD), malondialdehyde (MDA) and glutathione(GSH) and inflammatory cytokines interleukin-6(IL-6), interleukin-1β(IL-1β) and tumor necrosis factor-α (TNF-α) were detected by ELISA. The high mobility group protein B1(HMGB1), Toll like receptor 4(TLR4) and p-NF-κB p65 protein expression levels were detected by Western Blot.[Results] Compared with the control group, there were statistically significant differences between the model group and the control group (P<0.05) in increased lung dry-wet weight ratio, increased levels of SOD, MDA and GSH, increased levels of IL-6, IL-1β and TNF-α, and increased expression levels of HMGB1 and TLR4 proteins, and increased p-NF-κB p65/NF-κB p65. Compared with model group, by the Semen Lepidii water extraction liquid of 10 g/kg, 20 g/kg, 0.002 g/kg group and dexamethasone group reduced lung wet weight ratio, lung tissue pathology damage degree, SOD and GSH content increased, MDA content reduced, IL-6, IL-1β and TNF-α levels reduced, HMGB1, TLR4 protein expression level and p-NF-κB p65/NF-κB p65 ratio decreased, significant difference was statistically significant (P<0.05).[Conclusion] Semen Lepidii aqueous extract can improve the pathological injury, inhibit the release of inflammatory factors, improve oxidative stress, and inhibit the activation of HMGB1/TLR4/NF-κB signaling pathway in the rat model of inhalation lung injury induced by LPS. |
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