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| Mechanism and verification of Allii Fistulost Bulbus extract against nonalcoholic fatty liver disease based on network pharmacology |
| Hits 981 Download times 381 Received:January 11, 2022 |
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| DOI
10.11656/j.issn.1673-9043.2022.03.16 |
| Key Words
Allii Fistulost Bulbus extract;network pharmacology;nonalcoholic fatty liver disease |
| Author Name | Affiliation | E-mail | | LIU Yun | Hubei University of Traditional Chinese Medicine, Wuhan 430000, China | | | GUO Jie | Wuhan First Hospital Affiliated to Hubei University of Traditional Chinese Medicine, Wuhan 430000, China | | | ZHANG Manling | Hubei University of Traditional Chinese Medicine, Wuhan 430000, China | | | SHI Zhaohong | Wuhan First Hospital Affiliated to Hubei University of Traditional Chinese Medicine, Wuhan 430000, China | 2539861312@qq.com |
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| Abstract
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| [Objective] To investigate the potential targets of Allii Fistulost Bulbus in treatment of NAFLD based on network pharmacology, and SiRNA technology was used for verification in NAFLD rats.[Methods] The Traditional Chinese Medicine System Platform(TCMSP) database was used to find out active components and potential targets of Allii Fistulost Bulbus. Then the potential target information of NAFLD was retrieved by Genecards databases. Perl language software was used for preprocessing, and the R language software, Cytoscape software and String network data platform were used data analysis and picture production. In the end, we used SiRNA technology to inhibit the expression of PPARGC1A in the NAFLD rats to verify the curative effect.[Results] Two active components of kaempferol and catechin were screened out, and 2 important targets for the treatment of NAFLD with PPARGC1A and PPARG were predicted. The results of animal experiments confirm that compare to the model group, Allii Fistulost Bulbus extract can relieve liver steatosis, and up-regulated the protein expression of PPARGC1A and PPARG(P<0.01).[Conclusion] This study systematically predicted the potential target of Allii Fistulost Bulbus against NAFLD by Network Pharmacology and verified the feasibility to treat NAFLD by inducing PPARGC1A in animal experiment. |
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