|
| The protective effect and mechanism of eriodictyol on the liver injury in diabetic rats induced by high-fat diet |
| Hits 804 Download times 449 Received:January 11, 2022 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1673-9043.2022.03.17 |
| Key Words
eriodictyol;diabetes;apoptosis;TUNEL;immunohistochemistry;TNF-α |
| Author Name | Affiliation | | YANG Min | Department of Pediatrics, Shiyan Taihe Hospital, Shiyan 442099, China | | ZHANG Feng | Department of Pediatrics, Affiliated Hospital of Wuhan University of Science and Technology, Wuhan 430064, China | | FU Jiao | Department of Pediatrics, Affiliated Hospital of Wuhan University of Science and Technology, Wuhan 430064, China | | PI Zhijie | Department of Pediatrics, Affiliated Hospital of Wuhan University of Science and Technology, Wuhan 430064, China | | HE Li | Department of Pediatrics, Affiliated Hospital of Wuhan University of Science and Technology, Wuhan 430064, China |
|
| Abstract
|
| [Objective] To explore the protective effect of Eriodictyol on the liver injury of diabetes mellitus (DM) rats induced by high-fat diet, and the mechanism related to oxidative stress and insulin resistance.[Methods] The 50 male SD rats were divided into five groups, normal blank group, model group(DW), low-dose group(DW+Eriodictyol 2.5 mg/kg), medium-dose group(DW+Eriodictyol 5 mg/kg), high-dose group(DW+Eriodictyol 10 mg/kg), a diabetes model induced by high-fat diet combined with low-dose streptozotocin was established. Assess related indicators of insulin resistance, fasting blood glucose (FBG), fasting insulin (FINS) and insulin resistance index (HOMA-IR);Kit to detect the level of biochemical indicators of liver function, aspartate aminotransferase (AST), alanine aminotransferase (ALT), low-density lipoprotein (LDL), high-density lipoprotein cholesterol (HDL);HE staining to observe the pathological morphology of liver tissue;Kit to detect the level of oxidative stress markers, superoxide dismutase(SOD), malondialdehyde(MDA);TUNEL to observe the apoptosis of liver cells;Western blot to detect the expression of apoptosis-related proteins Bax and Bcl-2;immunohistochemistry to detect the level of TNF-α.[Results] Compared with the normal group, the rats in the model group showed the typical symptoms of DM "three more and one less", and the pathological inflammatory infiltration was serious. Various indicators suggested that the rats in the model group. Compared with the model group, the insulin resistance related indexes FBG, FINS, and HOMA-IR of the administration group decreased. The liver function indexes AST, ALT, LDL levels decreased, and the HDL level increased;oxidative stress related indexes, SOD activity increased, and MDA content decreased;The expression of apoptotic cells and related proteins Bax/Bcl-2 decreased and the positive expression of inflammatory factor TNF-α decreased.[Conclusion] Eriodictyol can improve diabetic liver injury induced by high-fat diet and plays a protective effect on the liver of DM rats. The mechanism may be related to the regulation of transaminase levels, promotion of lipid metabolism, reducing oxidative stress response, reducing protein expression related to hepatocyte apoptosis, and alleviating inflammatory infiltration of liver tissue. |
|
|
|
|
|
