|
| Simultaneous determination of eight compounds in Yinxu Weitong Granules by HPLC-QAMS |
| Hits 657 Download times 378 Received:December 20, 2021 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1673-9043.2022.03.18 |
| Key Words
Yinxu Weitong Granules;HPLC-QAMS;multi-index component;RCF |
| Author Name | Affiliation | E-mail | | CHEN Xiaocun | Dazhou Hospital of Integrated Traditional Chinese and Western Medicine, Dazhou 635000, China | | | WEI Tanjun | Dazhou Hospital of Integrated Traditional Chinese and Western Medicine, Dazhou 635000, China | hotq1323@163.com | | CHEN Fei | Dazhou Hospital of Integrated Traditional Chinese and Western Medicine, Dazhou 635000, China | | | XIAO Cheng | Dazhou Hospital of Integrated Traditional Chinese and Western Medicine, Dazhou 635000, China | | | CHEN Yong | Chengdu Diao Group Tianfu Pharmaceutical Co. Ltd., Dazhou 635027, China | |
|
| Abstract
|
| [Objective] To establish an HPLC-QAMS method for simultaneous determination of oxypaeoniflorin, albiflorin, paeoniflorin, benzoylpaeoniflorin, methylophiopogonanone A, methylophiopogonanone B, falcarindiol and panaxynol in Yinxu Weitong Granules, and to establish the multi-index components quality evaluation model for Yinxu Weitong Granules.[Methods] The Agilent HC C18 column was used, and the mobile phase consisted of acetonitrile-0.1% phosphoric acids solution with gradient elution, and the flow rate was 1.0 mL/min;The column temperature was 30℃;The detection wavelength were 230, 296 and 254 nm;The injection volume was 10 μL. With paeoniflorin as the internal reference, the relative correction factor(RCF) with other seven components was established, and the content of each component was calculated. The external standard method(ESM) was used to determine the contents of 8 components in Yinxu Weitong Granules to verify the accuracy of the calculation results of the QAMS.[Results] The 8 components have a good linear relationship within their respective ranges(r ≥ 0.999 1), whose average recoveries(RSD) were 98.90%(1.01%), 100.02%(0.94%), 99.52%(0.76%), 97.88%(1.35%), 99.14%(1.42%), 96.85%(0.65%), 97.83%(0.92%) and 98.65%(1.24%), respectively. There was no significant difference between HPLC-QAMS and ESM.[Conclusion] The established HPLC-QAMS method is convenient and accurate, which can be used for simultaneous determination of eight compounds in Yinxu Weitong Granules. |
|
|
|
|
|
