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| Effect of Gancao Yangyin Decoctionon improving psoriasis-like skin lesions and regulating inflammation in mice |
| Hits 602 Download times 345 Received:February 18, 2022 |
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| DOI
10.11656/j.issn.1673-9043.2022.04.12 |
| Key Words
animal model;imiquimod;poriasis;Treg/Th17;traditional Chinese medicine |
| Author Name | Affiliation | E-mail | | WU Huimei | Southern Medical Univerisity-Karolinska Institute United Medial Inflammation Center, College of Pharmacy, Southern Medical University, Guangzhou 510515, China | | | CHEN Yong | Department of Rheumatology and Immunology, Shenzhen People's Hospital, The Second Clinical Medical College of Jinan University, The First Affiliated Hospital of Southern University of Science and Technology, Shenzhen 518020, China
Department of Rheumatology, Integrated Hospital of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510315, China | | | XIAO Changhong | Department of Rheumatology, Integrated Hospital of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510315, China | nandakumar@smu.edu.cn | | NANDAKUMAR Kutty Selva | Southern Medical Univerisity-Karolinska Institute United Medial Inflammation Center, College of Pharmacy, Southern Medical University, Guangzhou 510515, China | |
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| Abstract
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| [Objective] To explore the efficacy of self-made Gancao Yangyin Decoction (GNY) on imiquimod (IMQ)-induced psoriasis-like inflammation.[Methods] The mice were divided into healthy control group, model group (IMQ-induced psoriasis mice) and treatment group(GNY concentration of 2.5 g/kg and 5 g/kg given to psoriasis mice. A total of 4 groups, each group has 8 mice. Psoriasis area and severity index (PASI), HE staining and Baker's scores of skin were used to assess the severity of the disease. Immunohistochemistry or immunofluorescence staining was used for detection of Gr-1, CD11c, RORγt and TGF-β in the skin. Flow cytometry was used to analyze Th17 cells and Treg cells expression in spleen. The relative mRNA expression of IL-6, IL-10, IL-17A, IL-22, IL-23, TNF-α, TGF-β and AMPK-1 were examined by qPCR.[Results] Compared with the model group, psoriasis treated by GNY are improve at both concentrations. The morphological characteristics of the psoriasis lesions include increasesd PASI scores and epidermis thickness, which can be reduced after GNY treatment; higher concentrations of GNY significantly reduced Gr-1, CD11c, RORγt and TGF-β positive cells expression in skin; Down-regulation of Th17 cells but increase of Treg cells was found in the spleen after GNY treatment. In addition, the increase of IL-6, IL-10, IL-17A, IL-22, IL-23, TNF-α, TGF-β and AMPKɑ1 induced by IMQ were significantly reduced after treatment of GNY with the concentration of 5 g/kg.[Conclusion] This study proved that GNY improve the inflammation induced by IMQ, which provides a basis for clinical treatment of psoriasis. |
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