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| Effect of paederosidic acid on inflammatory factor levels, growth, apoptosis, invasion and angiogenesis of hepatocellular carcinoma HepG2 cells |
| Hits 826 Download times 625 Received:March 05, 2022 |
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| DOI
10.11656/j.issn.1673-9043.2022.04.14 |
| Key Words
paederosidic acid;liver cancer;HepG2 cells;invasion;angiogenesis;VEGF |
| Author Name | Affiliation | E-mail | | DU Guoming | Department of General Surgery, Hebi People's Hospital, Hebi 458030, China | DGM13569635177@163.com | | WU Tao | Department of General Surgery, Hebi People's Hospital, Hebi 458030, China | | | LU Wenshi | Department of General Surgery, Hebi People's Hospital, Hebi 458030, China | | | LIU Zuoming | Department of General Surgery, Hebi People's Hospital, Hebi 458030, China | | | WANG Zhenyi | Department of General Surgery, The First Affiliated Hospital of Xinxiang Medical College, Xinxiang 453100, China | |
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| Abstract
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| [Objective] To investigate the effect of paederoside acid on the levels of inflammatory factors, growth, apoptosis, invasion and angiogenesis of hepatocarcinoma cell lines.[Methods] The viability of liver cancer cell lines were tested with CCK-8 method; Flow cytometry was applied to detect cell apoptosis; ELISA was used to detect the content of related inflammatory factors; Western blot was applied to detect Bax, Bcl-2, Caspase-3, IL-6, IL-10, E-cad, Vimentin, FN, VEGF; Cell invasion was detected with Transwell; Endothelial cell tube formation test was applied to test the formation of tumor tubules.[Results] When the concentration of paederoside acid was less than 20 μg/mL, there was no significant difference in the inhibitory effect on the viability of liver cancer cell lines; When the concentration was greater than 40 μg/mL at 48 h, the inhibitory effect on HepG2 was obvious (P<0.05), and the effect showed a dose-dependent inhibition; HepG2 cells were selected for 48h treatment in later experiments, and the experiment was divided into three dose groups (0, 20, 40 μg/mL), and erlotinib (0.86 ng/mL) was used as the positive control. Compared with the blank control group, the apoptosis rate, the level of anti-inflammatory factors (IL-10), and the expression of Bax/Bcl-2, Cleaved Caspase-3/Caspase-3 and E-cad protein in the 40 μg/mL group were significantly increased (P<0.05);The level of pro-inflammatory factors (IFN-γ, IL-6), the number of invasive cells and the expression of related proteins(Vimentin, FN), the number of tubules formed and the expression of vascular endothelial growth factor were significantly reduced(P<0.05).[Conclusion] Paederosidic acid regulates the level of inflammatory factors, inhibits the growth, invasion, tumor angiogenesis and promotes apoptosis of liver cancer cells HepG2, and alleviates the occurrence and development of liver cancer. |
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