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| Prunella vulgaris L. attenuates experimental autoimmune thyroiditis by inducing5-hydroxytryptamine expression and inhibiting Th17 T cell |
| Hits 527 Download times 375 Received:February 03, 2022 |
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| DOI
10.11656/j.issn.1673-9043.2022.04.16 |
| Key Words
autoimmune thyroiditis;5-hydroxytryptamine;5-HT transporter;Th17 |
| Author Name | Affiliation | E-mail | | ZHANG Hong | Department of General Practice, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, China | | | GENG Hui | Department of Pharmacy, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, China | | | QU Haili | Nursing Department, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, China | | | ZHONG Xia | Department of General Practice, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, China | zxbs1231@126.com |
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| Abstract
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| [Objective] Prunella vulgaris L. has been used in the treatment of autoimmune thyroiditis(AIT), but the mechanism is not clear. In this study, experimental autoimmune thyroiditis (EAT) rats model was built to study the effects of Prunella vulgaris L. aqueous extract on 5-hydroxytryptamine (5-HT), 5-HT transporter (SERT) and Th17.[Methods] The aqueous extract of Prunella vulgaris L. were extracted and analyzed by HPLC; The EATs were established by subcutaneous injection of thyroglobulin (Tg) combined with high iodine water. Then the rats were divided into model control group (EAT), low-dose Prunella vulgaris L. group (E-Low), medium dose Prunella vulgaris L. group (E-MID) and high-dose Prunella vulgaris L. group (E-HIG) for 12 weeks. The levels of serum thyroglobulin antibody (TgAb) and cytokines were detected by enzyme-linked immunosorbent assay (ELISA); thyroid morphology was observed by HE staining; The levels of CD4+ IL17A+ (Th17) lymphocytes in spleen were detected by flow cytometry; SERT level in spleen was detected by qRT-PCR; The levels of 5-HT in spleen and serum, IL-17A and IL-6 in serum were detected by ELISA.[Results] Rosmarinic acid is the main component of Prunella vulgaris L. aqueous extract. After 12 weeks of treatment, Prunella vulgaris L. significantly reduce serum TgAb (P<0.05 or P<0.01), thyroid volume (P<0.01), inflammatory infiltration in thyroid gland, Th17 ratio in Peripheral blood (P<0.01) and serum IL-17A, moreover, it effectively inhibited SERT level in spleen (P<0.01), and increased 5-HT in spleen and serum (P<0.01).[Conclusion] Prunella vulgaris L. can inhibit the differentiation of Th17 cells by increasing the 5-HT content in EAT rats to regulate immunity. This research provides a new idea for the treatment of EAT with Prunella vulgaris L. |
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