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| Study on anti-atherosclerotic effect of Ligustrum lucidum based on network pharmacology |
| Hits 583 Download times 290 Received:March 15, 2022 |
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| DOI
10.11656/j.issn.1673-9043.2022.04.18 |
| Key Words
Ligustrum lucidum;atherosclerosis;network pharmacology |
| Author Name | Affiliation | E-mail | | LYU Jialin | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | WANG Rongrong | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | HE Mingshuai | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | JIANG Miaomiao | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | miaomiaojiang@126.com |
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| Abstract
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| [Objective] To analyze the molecular biological mechanism of Ligustrum lucidum in the treatment of atherosclerosis based on network pharmacology and bioinformatics methods.[Methods] Acquire the Ligustrum lucidum active chemical ingredients based on traditional Chinese medicines systems pharmacology and literature. The targets of LLF were screened by uploading the chemical structure to PharmMapper database, establish "constituent-target" network. Screening atherosclerosis related targets through TTD, DrugBank and DisGeNET databases. The mutual target proteins was analyzed by STRING database to perform GO biological process analysis and KEGG pathway enrichment analysis. The antiatherosclerotic activity of salidroside and cilioside was evaluated by oil red O staining and intracellular total cholesterol detection.[Results] The "Constituent-Target" network display a total of 14 active chemical constituent and 170 potential disease targets were obtained, and there were 9 common targets of Ligustrum lucidum chemical components and atherosclerosis. GO biological process analysis identified 352 terms and the result of KEGG enrichment analysis showed 14 pathways were associated with atherosclerosis. Cell experiments showed that salidroside and acteoside significantly reduced the formation of foam cells and the content of intracellular total cholesterol.[Conclusion] The phenylethanol glycosides may play an anti-atherosclerosis role by regulating AMPK and PPAR signaling pathways to regulate cholesterol metabolism. |
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