|
| Salidroside down-regulates KRT17 and increases the sensitivity of paclitaxel to chemotherapy in ovarian cancer cells |
| Hits 671 Download times 455 Received:May 23, 2022 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1673-9043.2022.05.16 |
| Key Words
ovarian cancer;salidroside;paclitaxel;Keratin17 |
| Author Name | Affiliation | | LIU Hua | Obstetrics and Gynecology Department of Yicheng People's Hospital, Yicheng 441400, China | | ZHOU Lingling | Obstetrics and Gynecology Department of Yicheng People's Hospital, Yicheng 441400, China | | WEI Dandan | Obstetrics and Gynecology Department of Yicheng People's Hospital, Yicheng 441400, China | | XU Yiyan | Obstetrics and Gynecology Department of Yicheng People's Hospital, Yicheng 441400, China | | ZHANG Hui | Xiangyang Central Hospital, Obstetrics and Gynecology Department of Affiliated Hospital of Hubei University of Arts and Sciences, Xiangyang 441021, China |
|
| Abstract
|
| [Objective] Salidroside is a compound extracted from dried roots and rhizomes or dried whole grasses of large plants of the family rhodiola rosea. It has a variety of pharmacological effects. The aim of this study was to investigate the effect of salidroside's down-regulation of Keratin17(KRT17) on the chemotherapy sensitivity of paclitaxel to ovarian cancer cells.[Methods] SKOV-3 cells were treated with salidroside and paclitaxel alone or in combination. The cells were randomly divided into four groups:control group, salidroside alone, paclitaxel alone and in combination. Cell Counting Kit 8(CCK-8) was used to detect cytotoxicity, 5-acetyl-2'-deoxyuridine(EDU) was used to detect cell proliferation, and Hoechst 33258 was used to detect apoptosis. The formation of autophage was detected by immunofluorescence method, and the expression of KRT17 and autophagy related proteins were detected by Western Blot.[Results] Compared with DMSO group, individual treatment group increased with the concentration of salidroside, paclitaxel enhanced on SKOV-3 cell toxicity, number of cell proliferation, P62 protein expression decreased, cell apoptosis rate, the number of LC3 fluorescence spots, LC3-Ⅱ/LC3-I ratio, and Beclin1 expression were increased. Moreover, the synergistic effect of salidroside and paclitaxel was better than salidroside and paclitaxel alone, which may be achieved by salidroside down-regulating the expression of KRT17 protein.[Conclusion] Salidroside down-regulates KRT17 and increases the chemotherapy sensitivity of paclitaxel to ovarian cancer cells. |
|
|
|
|
|
