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| Simultaneous quantitative analysis of eight components in Guiqi Buxue Oral Liquid by HPLC-QAMS |
| Hits 627 Download times 346 Received:April 27, 2022 |
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| DOI
10.11656/j.issn.1673-9043.2022.05.17 |
| Key Words
HPLC-QAMS;Guiqi Buxue Oral Liquid;relative correction factor;lobetyolin;syringin;calycosin 7-O-β-D-glucopyranoside;ononin;9,10-dimethoxy-pterocarpane-3-O-β-D-glucoside;dehydrotumulosic acid;dehydropachymic acid;pachymic acid |
| Author Name | Affiliation | E-mail | | WANG Hualing | Department of Pharmacy, Traditional Chinese Medicine Hospital of Dezhou, Dezhou 253000, China | | | DENG Xia | Department of Pharmacy, Traditional Chinese Medicine Hospital of Dezhou, Dezhou 253000, China | | | REN Qiang | College of Pharmacy, Jining Medical University, Rizhao 276800, China | | | ZHOU Jinhui | College of Pharmacy, Jining Medical University, Rizhao 276800, China | bqu73304@163.com |
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| Abstract
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| [Objective] To establish an high performance liquid chromatography-quantitative analysis of multi-components by single marker(HPLC-QAMS) method for simultaneous determine of lobetyolin, syringin, calycosin 7-O-β-D-glucopyranoside, ononin, 9, 10-dimethoxy-pterocarpane-3-O-β-D-glucoside, dehydrotumulosic acid, dehydropachymic acid and pachymic acid in Guiqi Buxue Oral Liquid.[Methods] The determination was performed on an Agilent Zorbax SB-C18 column(250 mm×4.6 mm, 5 μm) with a gradient elution of acetonitrile-0.05% phosphoric acid as mobile phase at the flow rate of 1.0 mL/min. The detection wavelength were set at 254 nm for lobetyolin, syringin, calycosin 7-O-β-D-glucopyranoside, 9, 10-dimethoxy-pterocarpane-3-O-β-D-glucosideand ononin, 210 nm for dehydrotumulosic acid, dehydropachymic acid and pachymic acid. The column temperature was 25℃. Using calycosin 7-O-β-D-glucopyranoside as an internal standard, the relative correction factors of lobetyolin, syringin, calycosin 7-O-β-D-glucopyranoside, ononin, 9, 10-dimethoxy-pterocarpane-3-O-β-D-glucoside, dehydrotumulosic acid, dehydropachymic acid and pachymic acid were calculated, after which the content determination was made, and compared with the results of the external standard method(ESM) to verify the accuracy and feasibility of the method.[Results] Lobetyolin, syringin, calycosin 7-O-β-D-glucopyranoside, ononin, 9, 10-dimethoxy-pterocarpane-3-O-β-D-glucoside, dehydrotumulosic acid, dehydropachymic acid and pachymic acid showed good linear relationships within the ranges of 1.91~47.75, 0.64~16.00, 1.57~39.25, 0.98~24.50, 1.40~35.00, 0.76~19.00, 0.58~14.50, 1.16~29.00 μg/mL(r ≥ 0.999 1), whose average recoverie and relative standard deviation(RSD) were 98.2%(0.97%), 97.0%(1.05%), 100.1%(0.76%), 98.7%(1.32%), 99.1%(1.43%), 97.2%(0.89%), 97.3%(1.38%), 98.4(1.69%), respectively. No significant difference of quantitative results was observed between ESM and HPLC-QAMS method.[Conclusion] The HPLC-QAMS method can provide a supplement and improvement method for the existing quality standards of Guiqi Buxue Oral Liquid. |
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