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| Study on the protective effect of rhubarphol on ovalbumin induced asthmatic mice based on TWEAK/Fn14 signal pathway |
| Hits 641 Download times 371 Received:June 16, 2022 |
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| DOI
10.11656/j.issn.1673-9043.2022.05.19 |
| Key Words
rhubarphol;ovalbumin;asthma;TWEAK/Fn14 signal pathway;mouse |
| Author Name | Affiliation | | WANG Ting | Laboratory Department, Guangdong Second People's Hospital, Guangzhou 510317, China | | LI Haijian | Laboratory Department, Guangdong Second People's Hospital, Guangzhou 510317, China | | LIAO Ming | Laboratory Department, Affiliated Cancer Hospital of Guangzhou Medical University, Guangzhou 510095, China | | ZENG Wujiao | Laboratory Department, Guangdong Second People's Hospital, Guangzhou 510317, China |
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| Abstract
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| [Objective] To observe the protective effect of rhubarphol on asthmatic mice induced by ovalbumin (OVA), and explore whether the mechanism of its effect is related to TWEAK/Fn14 signal pathway.[Methods] Sixty C57BL/6 mice were randomly divided into the normal control group (CON group), the model group (OVA group), the dexamethasone positive control group (DEX group), the low dose rhubarphol group (RHU-L group), the medium dose rhubarphol group (RHU-M group) and the high dose rhubarphol group (RHU-H group) according to their body weight (10 mice/group). At the end of the experiment, asthma symptoms of each group were scored. Diff-quick staining method was used to measure the number of inflammatory cells in BALF of mice in each group. Histopathological changes of lung were observed by HE staining. The contents of IL-4, IL-5, TNF-α and IL-1β in BALF were detected by ELISA. Immunohistochemical method was used to detect the level of TWEAK in lung tissues of mice in each group. The expression levels of TWEAK and Fn14 were by Western blot in lung tissue.[Results] Rhubarphol could significantly reduce asthma symptom score (P<0.05), and decreased the total number of cells, eosinophils, neutrophils and lymphocytes in BALF of asthmatic mice (P<0.05). Rhubarphol improved pathological changes in lung tissue, and reduced the levels of IL-4, IL-5, TNF-α and IL-1β in BALF (P<0.05). Rhubarphol decreased the expression of TWEAK and Fn14 in lung tissues (P<0.05).[Conclusion] Rhubarphol has protective effect on OVA induced asthma mice, it may be related to regulation of TWEAK/Fn14 signal pathway. |
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