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| Study on the preventive effect of Longdan Xiegan Decoction on acute hepatic encephalopathy in rats |
| Hits 587 Download times 344 Received:September 01, 2022 |
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| DOI
10.11656/j.issn.1673-9043.2022.06.14 |
| Key Words
Longdan Xiegan Decoction;acute hepatic encephalopathy;TAA;blood ammonia |
| Author Name | Affiliation | E-mail | | WU Zijun | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | FAN Baishuang | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | Luo Jiayi | State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
Guangdong Pharmaceutical University, Guangzhou 510006, China | | | Wei Xin | Guangdong Pharmaceutical University, Guangzhou 510006, China | | | Li Siqin | Guangdong Pharmaceutical University, Guangzhou 510006, China | | | WANG Lili | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | HE Xin | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
Guangdong Pharmaceutical University, Guangzhou 510006, China | hexintn@163.com |
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| Abstract
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| [Objective] To investigate the preventive and therapeutic effects of Longdan Xiegan Decoction on acute hepatic encephalopathy induced by TAA in rats. [Methods] The SPF Wistar rats were randomly divided into normal group, model group, positive drug group (Lactulose) and Longdan Xiegan Decoction group, with 8 rats in each group. Normal group and model group were given 10 mL/kg normal saline, positive drug group was given 6 g/kg lactulose, and Longdan Xiegan Decoction group was given 7 g/kg Longdan Xiegan Decoction, once a day, for consecutive 7 d. Model of acute hepatic encephalopathy was established after the end of administration on the fifth day. Except for the normal group, other groups were intraperitoneally injected with TAA 300 mg/kg for two days. The brain function score and Morris water maze were used to evaluate the learning and memory ability of rats;the blood ammonia and pH of colon contents were measured;the organ coefficient of liver and brain, alanine aminotransferase (ALT), aspartate aminotransferase(AST), alkaline phosphatase(ALP), total bilirubin(TBiL) and liver pathological section were used to reflect the tissue injury;the leves of interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α) in serum and the the contents of gamma aminobutyric acid(GABA), glutamic acid(Glu) and malonaldehyde(MDA) in brain were detected by enzyme-linked immunosorbent assay(ELISA). [Results] Compared with the model group, the brain function score of Longdan Xiegan Decoction group was significantly increased;both the latencies to find a hidden plateform in the water maze and the annulus-crossing index had significantly difference;the blood ammonia and pH of colon contents decreased significantly(P<0.001); the organ coefficient of brain significantly increased (P<0.001), while that of liver showed no difference;the values of ALT, AST, ALP and TBiL decreased significantly (P<0.001);the inflammatory cytokines of IL-6 and TNF-α in serum were lower than those in model group;the histopathological injury and fibrosis of livers were improved;the levels of GBAB and MDA in rat brain decreased significantly, and Glu increased significantly(P<0.01). [Conclusion] Longdan Xiegan Decoction can improve the learning and memory ability of model rats, reduce the content of ammonia in vivo, and improve the liver injury. It can reduce the content of inflammatory factors in serum of model rats, regulate the levels of GABA, Glu and MDA in brain, and play a role in treating acute hepatic encephalopathy. |
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