|
| Pharmacodynamics study of Zanhua Lurong Pills in the treatment of kidney-yang deficiency model rats |
| Hits 654 Download times 321 Received:June 26, 2022 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1673-9043.2022.06.15 |
| Key Words
Zanhua Lurong Pills;kidney-yang deficiency model;pharmacodynamics |
| Author Name | Affiliation | E-mail | | GENG Xinyue | School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing100029, China | | | GAO Xiaoyan | School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing100029, China | gaoxiaoyan@bucm.edu.cn |
|
| Abstract
|
| [Objective] To investigate the intervention effect of Zanhua Lurong Pills on kidney-yang deficiency model animals. [Methods] The kidney-yang deficiency rat model was established by intramuscular injection of hydrocortisone. Female/male rats were randomly divided into normal control group,model group and model administration group,with six rats in each group. Evaluate the intervention effect of Zanhua Lurong Pills on kidney- yang deficiency model rats by observing and recording the morphological indexes such as hair color,body posture, urine volume and body weight of rats in each group;evaluate the morphological changes of the testicular tissue structure of the male rats,and comparing the organ indexes of the testicular,uterus,kidney and other organs,as well as changes of testosterone(T),estradiol(E2) and tumor necrosis factor (TNF-α). [Results] The rat model of kidney- yang deficiency was established by intramuscular injection of hydrocortisone for three weeks. After the administration of Zanhua Lurong Pills,the morphological characteristics of the kidney-yang deficiency rat were improved,such as dry and dull body hair,hunched back,chills,increased urine output,and weight loss.In the male model group,the number of seminiferous tubule cells increased,sperm cells and spermatogenesis increased,the number of stromal cells recovered and the pathological condition improved. Compared with the organ index of the model group,the organ index of testis and kidney in the male model group increased significantly(P<0.01),the organ index of kidney in the female model group also increased significantly(P<0.05),and the uterine index increased,but there was no significant difference. In addition,the content of T in serum of male model rats and the content of E2 in serum of female model rats were significantly higher than those in the model group (P<0.01). Compared with the female/male model group,the content of TNF-α in the serum of the female/male model administration group was significantly lower(P<0.01). [Conclusion] Zanhua Lurong Pills can improve a series of symptoms caused by kidney-yang deficiency.After the administration of Zanhua Lurong Pills,the morphological characteristics of the kidney-yang deficiency rat model were improved,such as dry and dull body hair,hunched back,chills,increased urine output, and weight loss. The testicular tissue lesions of the male model administration group were improved. At the same time,it can increase the organ index of testis,uterus and kidney and regulate the content of T,E2 and TNF-α in serum of kidney-yang deficiency rats. |
|
|
|
|
|
