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| Susceptibility tests of Staphylococcus aureus to natural products using the resazurin method |
| Hits 895 Download times 471 Received:September 09, 2022 |
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| DOI
10.11656/j.issn.1673-9043.2022.06.18 |
| Key Words
resazurin;minimum inhibitory concentration;Staphylococcus aureus;natural products;antibacterial activity |
| Author Name | Affiliation | E-mail | | CUI Naibo | School of Pharmaceutical Engineering of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | MA Jingqian | School of Pharmaceutical Engineering of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | YU Heshui | School of Pharmaceutical Engineering of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Municipal Experimental Teaching Demonstration Center of Traditional Chinese Medicine Pharmaceutical Engineering, Tianjin 301617, China | | | LI Zheng | School of Pharmaceutical Engineering of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
State Key Laboratory of Component Traditional Chinese Medicine, Tianjin 301617, China
Municipal Experimental Teaching Demonstration Center of Traditional Chinese Medicine Pharmaceutical Engineering, Tianjin 301617, China | | | HE Yongzhi | School of Pharmaceutical Engineering of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Municipal Experimental Teaching Demonstration Center of Traditional Chinese Medicine Pharmaceutical Engineering, Tianjin 301617, China | | | BIE Songtao | School of Pharmaceutical Engineering of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Municipal Experimental Teaching Demonstration Center of Traditional Chinese Medicine Pharmaceutical Engineering, Tianjin 301617, China | song9209@tjutcm.edu.cn |
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| Abstract
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| [Objective] A resazurin-based microtiter method was developed for the quantitative monitoring the inhibitory effects of rhein,gossypol,berberine hydrochloride,plumbagin and quercetin. [Methods] The conditions of resazurin assay were optimized by evaluating influences of the concentration of resazurin,the concertration of bacteria,the species of mediums and rection time on the optical density. The resazurin-based microtiter method was used to drug susceptibility tests to obtain the minimum inhibitory concentration(MIC),toxic regression equation, coefficient of association (R2) and semi-inhibitory concentration(IC50). The feasibility of high throughput drug screening was evaluated by Z value. [Results] The result showed the concentration of resazurin was 20 μg/mL,the medium was LB,the concertration of bacteria was 5×105CFU/mL,rection time was 9 h. The results obtained by the two methods were consistent. [Conclusion] Five kinds of natural products have a certain inhibitory effect. And the resazurin- based assay can be used successfully to assess antibacterial properties of natural products,along with the characteristics of good accuracy,high sensitivity and high throughput. |
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