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| Effects of taxifolin on autophagy,apoptosis and senescence of SiHa cells in cervical cancer through PI3K/AKT/mTOR pathway |
| Hits 741 Download times 345 Received:August 20, 2022 |
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| DOI
10.11656/j.issn.1673-9043.2022.06.20 |
| Key Words
cervical cancer;taxifolin;autophagy;apoptosis |
| Author Name | Affiliation | E-mail | | CHEN Haiyan | Department of Gynecology and Obstetrics, Zengdu Hospital, Suizhou 441300, China | | | ZENG Xueli | Department of Gynecology and Obstetrics, Zengdu Hospital, Suizhou 441300, China | zengxueliqqq@163.com |
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| Abstract
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| [Objective] To observe the effects of taxifolin on autophagy,apoptosis and senescence of SiHa cells in cervical cancer. [Methods] SiHa cells of cervical cancer were treated with 0,5,10 and 20 μmol/L Taxifolin. Cell proliferation multiple was measured by CCK-8 method. Beclin1,p62 and LC3II/LC3I were detected by western blot. LC3 + was detected by immunofluorescence assay. Flow cytometry was used to detect apoptosis. Mitochondrial membrane potential was detected by flow sorting. Protein expression levels of Bcl-2,Bax,Caspase-3,cleaved Caspase-3,Caspase-9,cleaved Caspase-9,PI3K,p-PI3K,AKT,p-AKT,mTOR and p-mTOR were detected by protein western blot,AKT activator SC79 was added for verification. [Results] Compared with taxifolin 0 μmol/L group,10,20 μmol/L taxifolin group cell proliferation ratio decreased significantly (P<0.05),p62 protein levels decreased significantly (P<0.05),Beclin1,LC3II/LC3I protein were significantly increased (P<0.05),LC3+ content increased significantly(P<0.05),the apoptosis rate increased significantly(P<0.05),significantly increased mitochondrial membrane potential (P<0.05),the Bcl-2/Bax ratio decreased significantly (P<0.05),The ratio of cleaved Caspase-3/Caspase-3 and cleaved Caspase-9/Caspase-9 was increased (P<0.05),and the ratio of p-PI3K/PI3K,p-AKT/AKT, and p-mTOR/mTOR were significantly decreased(P<0.05). The addition of SC79,a PI3K/Akt signaling pathway activator, could reverse the effects of taxifolin on apoptosis,autophagy and PI3K/AKT signaling pathway related protein expression in SiHa cells. [Conclusion] Taxifolin induces autophagy,apoptosis and senescence of SiHa cells in cervical cancer by inhibiting the activation of PI3K/AKT/mTOR pathway. |
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