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| Absorption properties of baicalin-berberine complex and nanocrystals in Caco-2 monolayer model |
| Hits 1154 Download times 1285 Received:September 17, 2022 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1673-9043.2023.01.17 |
| Key Words
baicalin;berberine;Caco-2 cell monolayer model;cumulative amount transported;Papp |
| Author Name | Affiliation | E-mail | | WANG Jilin | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | FENG Xiaojiao | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | DANG Wenli | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LI Ziwei | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LIU Zhidong | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | PI Jiaxin | State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | pijiaxin@tjutcm.edu.cn |
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| Abstract
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| [Objective] The absorption properties of Baicalin (BA)-Berberine (BBR) complex and nanocrystals were studied,and the improvement of nanocrystals technology on the absorption properties of BA-BBR complex was discussed.[Methods] The concentration of the BA-BBR complex and nanocrystals on Caco-2 cell-line were selected by CCK-8 method. The Caco-2 cell monolayer model was constructed to explore the membrane transport of BA-BBR complex and nanocrystals. The influence of P-gp inhibitor verapamil on the cumulative transport volume and apparent permeability coefficient (Papp) was further studied.[Results] Papp values of BA and BBR in the complex group were lower than those in the physical mixture group. Compared with the complex group,the cumulative amount transported and Papp values of BA and BBR in nanocrystals group were significantly increased. Papp values of BA and BBR in nanocrystals were 1.3 and 2.3 times than those in the complex group,respectively. After verapamil addition,Papp values of BA and BBR were increased by 1.5 and 2.0 times separately in BA-BBR complex,while not significantly increased in nanocrystals.[Conclusion] Unlike physical mixtures,BA-BBR complex exhibited low permeability and high susceptibility on P-gp efflux. Nanocrystals could achieve the significantly permeability improvement and efflux reduction of BA-BBR complex. |
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