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| Rapid determination of short chain fatty acids in leech by gas chromatography |
| Hits 604 Download times 888 Received:September 11, 2022 |
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| DOI
10.11656/j.issn.1673-9043.2023.01.19 |
| Key Words
leech;short chain fatty;gas chromatography |
| Author Name | Affiliation | E-mail | | HUANG Fei | State Key Laboratory of Component-based Chinese Medicine, Tianjin Key Laboratory of Traditional Chinese Medicine Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LI Lingyun | State Key Laboratory of Component-based Chinese Medicine, Tianjin Key Laboratory of Traditional Chinese Medicine Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | YU Huijuan | State Key Laboratory of Component-based Chinese Medicine, Tianjin Key Laboratory of Traditional Chinese Medicine Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | WANG Yuefei | State Key Laboratory of Component-based Chinese Medicine, Tianjin Key Laboratory of Traditional Chinese Medicine Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | CUI Ying | State Key Laboratory of Component-based Chinese Medicine, Tianjin Key Laboratory of Traditional Chinese Medicine Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | CHAI Xin | State Key Laboratory of Component-based Chinese Medicine, Tianjin Key Laboratory of Traditional Chinese Medicine Chemistry and Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | chaix0622@tjutcm.edu.cn |
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| Abstract
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| [Objective] A quantitative analysis method was established for determination of short chain fatty acids (SCFAs) (acetic acid,propionic acid,butyric acid,isobutyric acid,valeric acid,isovaleric acid,and hexanoic acid) in leech by gas chromatography.[Methods] The content of seven SCFAs in leech samples was determined by gas chromatography with 2-ethylbutyric acid and 2-ethylhexanoic acid as internal standards. The components were separated by DB-FFAP elastic quartz capillary column (30 m×0.25 mm×0.25 μm) by programmed rise of temperature, which were detected by hydrogen flame ionization detector. The temperature of inlet and FID detector was fixed at 240℃,respectively. The high purity nitrogen was employed as carrier gas (1.0 mL/min) and make-up gas (25 mL/min) respectively.[Results] The content of SCFAs from different batches leech was obviously different,and the total content of SCFAs was distributed from 1 101 to 8 571 μg/g. Furthermore,the content of acetic acid,propionic acid,isobutyric acid,valeric acid,isovaleric acid,and hexanoic acid was 783.0~3 479,246.7~5 166,0~87.73,0~73.02,0~112.8,0~65.65 μg/g,respectively.[Conclusion] The method established in this study for the determination of SCFAs in leech is simple,rapid and stable,which can provide basis for the quality control of leech. |
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