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| Study on the therapeutic effect and mechanism of Sini San on depression rats |
| Hits 570 Download times 840 Received:November 20, 2022 |
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| DOI
10.11656/j.issn.1673-9043.2023.02.14 |
| Key Words
Depression;Sini San;Neurotransmitter;ERK1/2-CREB-BDNF signal path |
| Author Name | Affiliation | E-mail | | FANG Zubing | Department of Psychological Rehabilitation, Wuhan Hankou Hospital, Wuhan 430012, China | | | YU Xuedan | Department of Psychological Rehabilitation, Wuhan Hankou Hospital, Wuhan 430012, China | | | HU Hongtao | Department of Psychological Rehabilitation, Shenzhen Kangning Hospital, Shenzhen 518020, China | | | DONG Hanning | Department of Psychological Rehabilitation, Wuhan Hankou Hospital, Wuhan 430012, China | 780541690@qq.com |
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| Abstract
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| [Objective] To investigate the effects of Sini San on behavior, central neurotransmitter and ERK1/2 creb-BDNF signaling pathway in depressed rats.[Methods] The experimental rats were randomly divided into normal group, model group, positive control group, Sini San low-dose group, Sini San medium-dose group and Sini San high-dose group, with 20 rats in each group. After administration, sugar water consumption experiment and open field experiment were carried out. Elisa kit was used to detect dopamine(DA), norepinephrine(NE) and 5-hydroxytryptamine(5-HT) in hippocampus of rats. The contents of neuropeptide Y(NPY), substance P(SP) and somatostatin(SS) in serum were detected by radioimmunoassay kit. Western blot was used to detect the expression of key proteins in erK1/2-CREb-BDNF signaling pathway in the cortex.[Results] Compared with normal group, sugar water preference and open field movement score in model group were significantly decreased(P<0.001);the contents of DA, NE and 5-HT in hippocampus were significantly down-regulated(P<0.001);NPY and SS in serum were significantly down-regulated (P<0.001);SP was significantly up-regulated(P<0.001). The phosphorylation levels of ERK1/2 and CREB proteins and the expression of BDNF protein were significantly down-regulated in cortex(P<0.001). Compared with model group, sugar water preference and open field motion score in Sini San administration group were significantly up-regulated(P<0.001);DA, NE and 5-HT contents in hippocampus were significantly up-regulated(All P <0.001), NPY and SS in serum were significantly up-regulated (P<0.001), SP was significantly down-regulated(All P<0.001). The phosphorylation levels of ERK1/2 and CREB protein and the expression of BDNF protein in cortex were significantly increased (P<0.01).[Conclusion] Sini San can up-regulate the neurotransmitter content in hippocampus, regulate the level of serum neuropeptide, promote phosphorylation and activation of ERK1/2-CREB-BDNF signaling pathway, and play the function of improving depression-like behavior in rats. |
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