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| Effect of Fuzheng Sanjie Recipe on H22 liver ascitic tumor mice and its immunological regulation |
| Hits 453 Download times 811 Received:February 09, 2023 |
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| DOI
10.11656/j.issn.1673-9043.2023.03.11 |
| Key Words
Fuzheng Sanjie Recipe;malignant ascite;tumor immune microenvironment;positive regulation |
| Author Name | Affiliation | E-mail | | HAN Jiatong | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300381, China | | | TIAN Fei | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China | 865708729@qq.com | | LIN Fangyuan | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300381, China | | | LIN Pengzhan | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300381, China | |
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| Abstract
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| [Objective] To investigate the therapeutic effect and immunomodulatory effect of Fuzheng Sanjie Recipe combined with cisplatin on H22 ascites tumor mice. [Methods] The H22 ascites tumor model was established,and 40 mice were randomly divided into a blank group,a tumor-bearing control group,a cisplatin group,and a traditional Chinese medicine(TCM) combined cisplatin group. The abdominal circumference and ascites volume of the mice were measured every day,and after continuous administration for 2 weeks,and blood was collected and the mice were sacrificed. Weigh the tumor,calculate the tumor inhibition rate,compare the weight of each organ of each group of mice,and determine the serum tumor necrosis factor-α(TNF-α),interferon-γ(IFN-γ) and white blood cells in the serum of mice by ELISA method Interleukin-2(IL-2) content. [Results] The body weight and abdominal circumference of the mice in the tumor-bearing group increased rapidly compared with the blank control group, a large amount of ascites was produced,and the weight of the thymus was reduced. Compared with mice in the cisplatin group,the weight and abdominal circumference of the mice in the Chinese medicine combined with cisplatin group increased slowly,the amount of ascites decreased,and the weight of the thymus increased. Compared with the tumor-bearing control group,the cisplatin group and the TCM combined with cisplatin group had anti-tumor effect,and the tumor inhibition rate of the TCM combined with cisplatin group was significantly better than that of the cisplatin group(P<0.05). The serum levels of TNF-α,IFN-γ and IL-2 in tumor-bearing mice in each group were lower than those in the blank control group(P<0.05). Compared with the cisplatin group,the levels of Chinese medicine combined with IFN-γ and IL-2 were significantly increased(P<0.05). [Conclusion] Fuzheng Sanjie Recipe can reduce the amount of ascites in mice with hepatoma H22 ascites,through up regulating the levels of TNF-α、IFN-γ and IL-2 in serum,we can promote the positive regulation of tumor immune microenvironment,improve the immune function of mice,and effectively inhibit the growth of tumor. |
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