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| UPLC-Q-Exactive Orbitrap-MS method analysis of the flavonoids in the aerial part of Glycyrrhiza glabra L. |
| Hits 505 Download times 737 Received:February 22, 2023 |
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| DOI
10.11656/j.issn.1673-9043.2023.03.12 |
| Key Words
aerial part of Glycyrrhiza glabra L.;chemical constituent of flavonoids;UPLC-Q-Exactive Orbitrap-MS analysis technology |
| Author Name | Affiliation | E-mail | | ZHANG Zhi | College of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China | | | YUN Lu | College of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China | | | HOU Bingyan | College of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China | | | LIU Ying | College of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China | | | JIA Qi | College of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China | | | WANG Wenquan | College of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China
Institute of Medicinal Plants, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China
Engineering Research Center for Standardized Production of Traditional Chinese Materia Medica, Ministry of Education, Beijing 102488, China | | | HOU Junling | College of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China
Engineering Research Center for Standardized Production of Traditional Chinese Materia Medica, Ministry of Education, Beijing 102488, China | mshjl@126.com | | PENG Yifeng | Xinjiang Quan An Pharmaceutical Co., Korla 841011, China | chinn203@126.com |
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| Abstract
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| [Objective] The flavonoid chemical constituents in the aerial parts(stems and leaves) of Glycyrrhiza glabra L. were analyzed to provide a scientific basis for the further development and utilization of the aerial part of Glycyrrhiza glabra L. and the clarification of its medicinal material basis. [Methods] The alcohol-extracted samples of the aerial parts of Glycyrrhiza glabra L. were precipitated and divided into water-soluble and fat-soluble parts. Samples were tested by ultra-high performance liquid chromatography coupled with hybrid quadrupole-orbitrap mass spectrometry(UPLC-Q-Exactive Orbitrap-MS). Data was processed and analyzed using Xcalibur software,and the resulting component molecular mass and multilevel map fragment information were identified in document,database and standards. [Results] A total of 100 flavonoids were detected from the aerial parts of Glycyrrhiza glabra L.,and 97 species were found for the first time in the aerial parts of Glycyrrhiza glabra L.. Among them,61 species were detected in water-soluble samples,and 69 species were detected in fat-soluble samples. [Conclusion] Through UPLC-Q-Exactive Orbitrap-MS liquid chromatography-mass spectrometry technology,a rapid and accurate qualitative method was established for the aerial part of Glycyrrhiza glabra L.,and it was confirmed that the content of flavonoids was abundant,which provided a basis for further development and utilization. |
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