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| Analysis of volatile oil from raw and processed hemp seed by GC-MS |
| Hits 504 Download times 376 Received:January 19, 2023 |
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| DOI
10.11656/j.issn.1673-9043.2023.03.14 |
| Key Words
hemp seed;processing;volatile oil;GC-MS |
| Author Name | Affiliation | E-mail | | LIU Jiangjing | Heilongjiang Academy of Chinese Medicine Sciences, Harbin 150036, China | | | ZHUANG Yan | Heilongjiang Academy of Chinese Medicine Sciences, Harbin 150036, China | | | LIU Huashi | Heilongjiang Academy of Chinese Medicine Sciences, Harbin 150036, China | | | LI Mengxue | Heilongjiang Academy of Chinese Medicine Sciences, Harbin 150036, China | | | HUO Jinhai | Heilongjiang Academy of Chinese Medicine Sciences, Harbin 150036, China | | | WANG Weiming | Heilongjiang Academy of Chinese Medicine Sciences, Harbin 150036, China | zyyjy@163.com |
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| Abstract
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| [Objective] To analyze the chemical composition of volatile oil from raw and processed hemp seed,and look for chemical components that change significantly before and after processing.[Methods] The volatile oil of raw and processed hemp seed was extracted by steam distillation,its chemical components were analyzed by GC-MS,and the relative contents of each component were calculated by internal standard method. OPLS-DA analysis and pair T test analysis were used to analyze the components before and after processing. VIP value and P value were used to screen out significant difference components.[Results] A total of 43 chemical constituents were identified. 40 compounds were identified from the volatile oil of raw hemp seed and 36 compounds were identified from the volatile oil of processed hemp seed. The 11 compounds with significant differences were screened,among which the contents of hexanal,2-pentylfuran,Anethole,trans,trans-2,4-Decadien-1-al increased after processing,while the contents of β-elemene,α-farnesene,Δ-cadinene,ar-Turmerone,Hexahydrofarnesyl acetone,Methyl palmitate, Methyl linolenate decreased after processing.[Conclusion] The composition of volatile oil was almost the same,but the content of volatile oil changed greatly. This study provided material basis for the formulation of processing standard of hemp seed,and provided reference for clinical application of hemp seed. |
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