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| Study on simultaneous determination of four index components in Jichuan Decoction and it's material standard technology |
| Hits 510 Download times 755 Received:March 06, 2023 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1673-9043.2023.04.12 |
| Key Words
classical prescription;Jichuan Decoction;material standard;quality control;preparation process |
| Author Name | Affiliation | E-mail | | LIU Jingjing | Engineering Center of Modern Chinese Medicine Discovery and Preparation Technology, Ministry of Education, Tianjin University of Chinese Medicine, Tianjin 301617, China
State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | QI Dongli | Engineering Center of Modern Chinese Medicine Discovery and Preparation Technology, Ministry of Education, Tianjin University of Chinese Medicine, Tianjin 301617, China
State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | GAO Wenyuan | Tianjin Key Laboratory of Modern Drug Delivery and High Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China | biochemgao@163.com | | LIU Zhidong | Engineering Center of Modern Chinese Medicine Discovery and Preparation Technology, Ministry of Education, Tianjin University of Chinese Medicine, Tianjin 301617, China
State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | liuzhidong@tjutcm.edu.cn |
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| Abstract
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| [Objective] To establish a method for the fingerprint and quantitative analysis of four components (naringin,neohesperidin,β-ecdysterone,and echinacoside) in Jichuan Decoction by HPLC. At the same time,the material reference technology research method was investigated to provide reference for scientific evaluation and quality control.[Methods] The HPLC analysis was performed on an Agilent ZORBAX SB-C18 column(4.6 mm×250 mm,3.5 μm) in a gradient elution program with acetonitrile-0.1% phosphoric acid solution as the mobile phase. The column temperature was set at 30℃,andthe flow rate was1.0 mL/min. The preparation process method of Jichuan Decoction was investigated to selectthe best process research method.[Results] A common pattern of HPLC fingerprint of Jichuan Decoction was established. Compared with the common pattern,the similarities of 12 batches of samples were above 0.950 with 14 common peaks,and six of which were identified and assigned. The contents of naringin,neohesperidin,β-ecdysterone,and echinacoside all within the range of 3.46 to 5.87 mg/g,3.35 to 5.36 mg/g,0.19 to 0.23 mg/g and 0.72 to 7.02 mg/g,respectively. The results of methodology study also showed that all indexes meet the requirements of content determination. The material reference technology research method wasas follows:accurately weighed the prescription amount of medicinal materials,added 300 mL of ultra-pure water,soaked for 45 min,boiled after boiling,covered and decocted to 130 to 140 mL,filtered with three layers of gauze while hot,twisted the slag,and let cool,The filtrate was concentrated under reduced pressure at 70℃ and freeze-dried to obtain the Jichuan Decoction material reference dry paste powder.[Conclusion] The established common pattern of HPLC fingerprint of Jichuan Decoction has good characteristics. Combined with the content determination of multiple index components,the material reference technology research methods are also conductive to promote the quality control of the preparation of Jichuan Decoction. |
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