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| Study on the effect of total flavonoids purified from Gynura Divaricate on rats with acute hyperuricemia |
| Hits 494 Download times 727 Received:March 08, 2023 |
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| DOI
10.11656/j.issn.1673-9043.2023.04.13 |
| Key Words
Gynura Divaricate;total flavonoids;hyperuricemia;reduce uric acid |
| Author Name | Affiliation | E-mail | | BAI Yuchao | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | XU Qiushuang | SAFE Pharmaceutical Technology Company, Beijing 100176, China | | | YU Feng | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | XU Kaige | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LI Tianxiang | Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | litianxiang612@sina.com |
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| Abstract
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| [Objective] In order to investigate the therapeutic effect of total flavonoids purified from Gynura Divaricate on acute hyperuricemia(HUA) in rats.[Methods] Sixty male Wistar rats were randomly divided into 6 groups:blank group,model group,allopurinol group,low-dose group of total flavonoids of Gynura Divaricate,medium dose group of total flavonoids of Gynura Divaricate,and high-dose group of total flavonoids of Gynura Divaricate. The acute HUA model of rats was established by the combined use of potassium oxazinate and hypoxanthine. One hour after modeling,allopurinol group was given allopurinol suspension(25 mg/kg) by gavage, and all administration groups of total flavonoids of Gynura Divaricate:low dose group(45 mg/kg),medium dose group(90 mg/kg),high dose group(180 mg/kg) were given corresponding concentrations of total flavonoids of Gynura Divaricate extract suspension by gavage once a day for 7 days. The changes of body mass and food intake of rats were recorded daily. The contents of Blood Uric Acid(BUA),Serum Creatinine(SCR),Blood Urea Nitrogen(BUN) and the activities of Adenosine Deaminase(ADA) and liver Xanthine Oxidase(XOD) were compared. The left kidney of rats was embedded in paraffin and stained with Hematoxylin Eosin(HE) to observe the pathological changes of rat kidney.[Results] Compared with the blank group,BUA in the model group increased significantly(P<0.05). Compared with the model group,the activity levels of BUA,BUN,SCr and XOD of rats in the low,medium and high dose groups of total flavonoids of Gynura Divaricate were significantly lower(P<0.05),and the activity of ADA of rats in the low and medium dose groups of total flavonoids of Gynura Divaricate was significantly lower(P<0.05). Compared with the model group,each administration group of total flavonoids of Gynura Divaricate can improve the glomerular atrophy,renal tubular dilatation and renal interstitial fibrosis of acute HUA model rats.[Conclusion] The total flavonoids of Gynura Divaricate can effectively reduce the level of HUA in rats and slow down the liver and kidney injury caused by HUA modeling agent. It has potential development value in the treatment of HUA. |
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