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| Investigate the effect of icariin on proliferation and osteogenic differentiation of human apical papillary stem cells |
| Hits 453 Download times 772 Received:March 08, 2023 |
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| DOI
10.11656/j.issn.1673-9043.2023.04.14 |
| Key Words
ICA;hSCAPs;osteolineage differentiation;cell proliferation |
| Author Name | Affiliation | | XIE Erting | Department of Orthodontics, Shenzhen Longhua District People's Hospital, Shenzhen 518109, China | | HUANG Bailan | Department of Periodontology, Shenzhen Longhua District People's Hospital, Shenzhen 518109, China | | LI Wenlong | Department of Oral and Maxillofacial Surgery, Shenzhen Longhua District People's Hospital, Shenzhen 518109, China | | LI Xiaoyu | Department of Oral and Maxillofacial Surgery, Shenzhen Longhua District People's Hospital, Shenzhen 518109, China |
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| Abstract
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| [Objective] To analyze the mechanism of human apical dental papilla stem cells(hscaps) proliferation and osteo differentiation after stimulation by Icariin(ICA).[Methods] The hSCAPs were incubated with different concentrations of ICA after cultured in vitro to the third passage for 2 d,and the osteo differentiation related factor osteoprotegerin of hSCAPs was detected at the mRNA and protein levels by QRT PCR and Western blotting,respectively (OPG) and nuclear factor-κ B receptor activator ligand (RANKL) expression;and the proliferation of hSCAPs incubated with different concentrations of ICA for different times was detected by CCK-8.[Results] After the isolation of hSCAPs from apical papilla in vitro,the expression of stem cell surface markers CD31,CD90,and CD146 cell expression rate was 4.03%,96.18%,and 95.42%,respectively,as examined by flow staining. Compared with the control group,In vitro stimulation with ICA significantly promoted the expression of OPG(P<0.05),decreased the mRNA transcription and translation of RANKL(P<0.05),and promoted the proliferation and differentiation of hSCAPs(P<0.05). The effect of ICA on the regulation of hSCAPs was the strongest when the concentration was 0.1 mol/L.[Conclusion] The promoting effects of ICA on cell proliferation and bone to differentiation of hSCAPs provide theoretical guidance for the clinical use of hSCAPs for dental disease treatment. |
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