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| GABAergic neurons in vlPAG downregulates visceral hypersensitivity by electroacupuncture in IBS mice |
| Hits 357 Download times 407 Received:June 30, 2023 |
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| DOI
10.11656/j.issn.1673-9043.2023.05.09 |
| Key Words
electroacupuncture;optogenetics;vlPAG;GABA;irritable bowel syndrome;visceral pain |
| Author Name | Affiliation | E-mail | | WANG Xinyue | Shanghai University of Traditonal Chinese Medicine, Shanghai 201203, China
Shanghai Research Institute of Acupuncure and Meridian, Shanghai 200030, China | | | WENG Zhijun | Shanghai Research Institute of Acupuncure and Meridian, Shanghai 200030, China | | | LIU Huirong | Shanghai Research Institute of Acupuncure and Meridian, Shanghai 200030, China | | | WU Luyi | Shanghai University of Traditonal Chinese Medicine, Shanghai 201203, China | | | HAN Dong | Shanghai University of Traditonal Chinese Medicine, Shanghai 201203, China
Shanghai Research Institute of Acupuncure and Meridian, Shanghai 200030, China | | | CHAI Jing | Shanghai University of Traditonal Chinese Medicine, Shanghai 201203, China
Shanghai Research Institute of Acupuncure and Meridian, Shanghai 200030, China | | | PENG Yang | Shanghai University of Traditonal Chinese Medicine, Shanghai 201203, China
Shanghai Research Institute of Acupuncure and Meridian, Shanghai 200030, China | | | MA Jindan | Shanghai Research Institute of Acupuncure and Meridian, Shanghai 200030, China | | | WU Huangan | Shanghai Research Institute of Acupuncure and Meridian, Shanghai 200030, China | | | ZHANG Fang | Shanghai University of Traditonal Chinese Medicine, Shanghai 201203, China
Shanghai Research Institute of Acupuncure and Meridian, Shanghai 200030, China | 869732219@qq.com |
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| Abstract
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| [Objective] Taking the mouse model of irritable bowel syndrome(IBS) as an example,optogenetic technology was used to regulate GABAergic neurons in ventrolateral periaqueductal gray(vlPAG),and the mechanism of GABAergic neurons in vlPAG participating in electroacupuncture to relieve visceral pain in IBS mice was discussed. [Methods] Vgat-Cre mice were randomly divided into:1) normal group(N):no treatment was given,only the same fixation as the electroacupuncture group was given;2) model group (M):do not do any treatment,only give the same fixation as the electroacupuncture group;3) model+eNpHR group(M+eNpHR):injection of rAAV2/9-EF1α-DIO-eNpHR3.0-EGFP virus,yellow light stimulation (20 Hz,5 ms,4 mW);4) model+ChR2 group (M+ChR2):injection of rAAV2/9-EF1α-DIO-hChR2-EGFP virus,blue light stimulation(20 Hz,5 ms,4 mW);5) electroacupuncture group(EA):acupuncture:(double),dense wave,frequency 2/100 Hz,current 1 mA,30 min/time,once a day,continuous treatment for 7 d. A model of chronic visceral pain of IBS was prepared by enema with 2,4,6-trinitrobenzen sulfunic acid. After the model was successfully prepared by general observation and abdominal wall withdrawal reflex score,the GABAergic neurons of vlPAG were activated or inhibited by optogenetic technology,and the changes of AWR scores in each group of mice were analyzed. [Results] 1)Compared with the normal group,the AWR score of mice in the model group increased(P<0.01);2) compared with before giving light,the AWR score of mice in the model+eNpHR group increased(P<0.05) when given yellow light to inhibit GABAergic neurons;the model+ChR2 group decreased when blue light activated GABAergic neurons (P<0.05);3) compared with the normal group, the AWR score of mice in the model group increased(P<0.01),and compared with the model group,the AWR score of the model+eNpHR group increased(P<0.05). The AWR score of the model+ChR2 group decreased(P<0.05). The AWR score decreased in the electroacupuncture group(P<0.05). [Conclusion] Optogenetic technology can effectively regulate GABAergic neurons in vlPAG brain regions,and the pain behavior effect of electricity on IBS mice is consistent with the effect trend of optogenetic activation of GABAergic neurons,indicating that electroacupuncture may play a role in alleviating visceral pain in IBS mice by activating GABAergic neurons in vlPAG. |
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