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| Explore the mechanism of Tongguan Zhuyun Decoction in the treatment of tubal obstructive infertility rats based on NF-κB signal pathway |
| Hits 398 Download times 528 Received:June 20, 2023 |
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| DOI
10.11656/j.issn.1673-9043.2023.05.10 |
| Key Words
Tongguan Zhuyun Decoction;tubal obstructive infertility;NF-κB;IκB;COX-2;PGE2 |
| Author Name | Affiliation | E-mail | | LI Panpan | Chengde Medical University, Chengde 067000, China | | | GAO Hui | Affiliated Hospital of Chengde Medical University, Chengde 067000, China | gaohuihbcd@163.com | | LIU Yulan | Affiliated Hospital of Chengde Medical University, Chengde 067000, China | | | HU Youqin | Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine, Cangzhou 061000, China | | | WU Siyi | Chengde Medical University, Chengde 067000, China | | | ZHANG Qingshuang | Chengde Medical University, Chengde 067000, China | |
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| Abstract
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| [Objective] To explore the mechanism of Tongguan Zhuyun Decoction in the treatment of tubal obstructive infertility rats based on NF-κB signal pathway. [Methods] The 140 female SD rats were randomly divided into normal group,model group,Chinese patent medicine group,Western medicine group and Tongguan Zhuyun Decoction high,medium and low dose groups,with 20 rats in each group. Except the normal group,the other groups were injected with staphylococcus aureus to establish the rat model of tubal obstructive infertility. On the 7th day after modeling,each group was given physiological salt water,Danhuang Quyu Decoction suspension,human placental tissue fluid,Tongguan Zhuyun Decoction high,medium and low dose were treated for 21 d,the rats in the pharmacodynamic group were sampled and treated,and the rats in the reproductive group were used for the male female cage experiment. ELISA was used to detect the levels of NF-κB,IκB,COX-2 and PGE2 in serum of rats in each group. The protein and gene expression levels of NF-κB,IκB,COX-2 and PGE2 were detected by Western blot and RT-PCR. The pathomorphological changes of fallopian tube were observed by naked eye and light microscope. Observe and record the pregnancy and delivery of rats in the reproductive group. [Results] 1) Compared with the normal group,the expression levels of NF-κB,COX-2,PGE2 protein and gene in the fallopian tube of the model group increased significantly,the expression levels of IκB protein and gene decreased significantly(P<0.05);compared with the model group,the expression levels of NF-κB,COX-2,PGE2 protein and gene in rat fallopian tube tissue of Tongguan Zhuyun Decoction high,medium and low dose groups decreased significantly,the expression levels of IκB protein and gene increased significantly(P<0.05);compared with Chinese patent medicine group and Western medicine group,the expression levels of NF-κB,COX-2,PGE2 protein and gene in rat fallopian tube tissue of Tongguan Zhuyun Decoction high-dose group decreased significantly,the expression levels of IκB protein and gene increased significantly(P<0.05). 2) After visual observation and light microscope observation,compared with the model group,the tissue morphology of fallopian tube in each administration group was improved in varying degrees,especially in the high-dose group of Tongguan Zhuyun Decoction,which was basically close to normal. 3) Effect on reproductive function:the pregnancy rate was 0% in the model group and 100% in the normal group, there is a statistical difference between the two groups(P<0.05). This shows that the method of staphylococcus aureus inoculation can successfully establish the rat model of oviduct obstructive infertility. The pregnancy rate and average litter size of the high,middle and low dose groups of Tongguan Zhuyun Decoction are statistically different from those of the model group(P<0.05). This shows that Tongguan Zhuyun Decoction has a definite effect on infertility caused by fallopian tube obstruction. [Conclusion] Tongguan Zhuyun Decoction can inhibit the expression of the pro-inflammatory factor NF-κB,COX-2 and PGE2 in SOI model rats,promotes the expression of anti-inflammatory factor IκB playing an anti-inflammatory and anti-adhesion role. The mechanism of treatment may be related to blocking NF-κB signal transduction pathway. |
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