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| Effect of oridonin on severe acute pancreatitis and tissue damage induced by ranin in rats |
| Hits 355 Download times 587 Received:April 28, 2023 |
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| DOI
10.11656/j.issn.1673-9043.2023.05.15 |
| Key Words
oridonin;severe acute pancreatitis;tissue damage |
| Author Name | Affiliation | E-mail | | FANG Ping | Department of General Surgery, Jingzhou Hospital of Traditional Chinese Medicine, Jingzhou 434002, China | | | DENG Xueli | Operating Room, Jingzhou Hospital of Traditional Chinese Medicine, Jingzhou 434002, China | dengxuelia@126.com | | DU Fangfang | Department of Orthopedics, Jingzhou Hospital of Traditional Chinese Medicine, Jingzhou 434002, China | | | PENG Qi | Department of General Surgery, Jingzhou Hospital of Traditional Chinese Medicine, Jingzhou 434002, China | | | ZHANG Li | Department of General Surgery, Minda Hospital Affiliated to Hubei University for Nationalities, Enshi 445000, China | |
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| Abstract
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| [Objective] To explore the regulatory role of oridonin in severe acute pancreatitis of immune disorders and tissue damage in rats. [Methods] Fifty SD male rats were randomly divided into healthy control group,model group(SAP),SAP+Ori5 mg/kg group,SAP+Ori10 mg/kg group,SAP+Ori20 mg/kg group,10 rats in each group. A rat model of severe acute pancreatitis was established by chemical induction,and acute pancreatitis was induced by subcutaneous injection of caerulein(25 μg/kg). Calculate pancreatic weight index,measure serum lipase and amylase levels by enzyme-linked immunosorbent assay(ELISA),observe the pathological changes of pancreatic tissue by hematoxylin-eosin(HE) staining;observe the expression of caspase-3 protein by immunohistochemistry;Western Blot detection of Bax/Bcl-2 protein expression;ELISA detection of serum tumor necrosis factor-α(TNF-α),inducible nitric oxide synthase(iNOS),interleukin 10(IL-10) expression levels;RT-PCR was used to detect the expression levels of interleukin-1β(IL-1β) and interleukin-4(IL-4) mRNA in pancreatic tissue. [Results] Compared with the control group,the pancreatic tissue function of the model group was significantly decreased(P<0.05),the tissue morphology was disordered,Caspase-3 protein was highly expressed,and the apoptosis index(Bax/Bcl-2) was significantly increased(P<0.05). TNF-α,iNOS,IL-10 protein were highly expressed in serum(P<0.05),and IL-1β and IL-4 mRNA were highly expressed in pancreatic tissue(P<0.05). Compared with the model group,there was no significant difference in pancreatic tissue function after 5 mg/kg oridonin was administered;10 and 20 mg/kg oridonin significantly improved pancreatic tissue function(P<0.05) and decreased apoptosis rate,down-regulation of caspase-3,bax protein expression,up-regulation of Bcl-2 protein expression(P<0.05),inhibition of pro-inflammatory factors TNF-α,iNOS protein expression and IL-1β mRNA expression(P<0.05),and promote anti- Inflammatory factor IL-10 protein expression and IL-4 mRNA expression(P<0.05). [Conclusion] Oridonin inhibits the pathological damage of pancreatic tissue,the expression of apoptotic proteins and the secretion of pro-inflammatory factors,and alleviates the immune disorder and tissue damage in rats with severe acute pancreatitis. |
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