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| Exploration of the mechanism of acupuncture treatment for ischemic stroke based on network pharmacology and metabolomics |
| Hits 644 Download times 807 Received:August 18, 2023 |
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| DOI
10.11656/j.issn.1673-9043.2023.06.10 |
| Key Words
ischemic stroke;acupuncture;metabolomics;network pharmacology;molecular docking |
| Author Name | Affiliation | E-mail | | ZHANG Yintao | Graduate School, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LIU Xinyu | College of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | WANG Yuyu | College of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | GUO Tao | College of Acupuncture and Tuina, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | ZHANG Fangfang | College of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | QIU Jiwen | College of Acupuncture and Tuina, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | qiujiwen2012@163.com | | WANG Yuming | College of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | wangyuming@tjutcm.edu.cn |
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| Abstract
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| [Objective] Combined with network pharmacology and metabolomics,the mechanism of acupuncture and moxibustion in the treatment of ischemic stroke was analyzed.[Methods] We use the Swiss Target Prediction database and Gene Cards database to search for the target of action;Construct a drug component target network using Cytoscape 3.9.1 software;STRING database obtains PPI network;Finally,the David platform was used for KEGG signaling pathway enrichment analysis. Ultra high-performance liquid chromatography quadrupole time of flight mass spectrometry (UPLC-Q-TOF/MS) was used to analyze the metabolic profile changes of rats in each group,and relevant metabolic pathways were queried using the Metabo Analyst database.[Results] A total of 49 serum based metabolic biomarkers were selected from the sham surgery group and model group (P＜0.05),with the main metabolic pathways being phospholipid metabolism,linoleic acid metabolism α- Linolenic acid metabolism,ether lipid metabolism,lysine degradation,arachidonic acid metabolism,which mainly contain metabolites such as phosphatidylcholine,Lys phosphatidylcholine,fatty acids,and phosphatidylethanolamine.[Conclusion] Acupuncture and moxibustion therapy may improve cerebral vascular microcirculation,promote angiogenesis,reduce lipid deposition,slow down inflammatory reaction,and improve body immunity through multiple targets and pathways. Combined with literature data,it is believed that acupuncture and moxibustion treatment has the characteristics of multiple components,multiple goals,and multiple paths,and has a certain effect on ischemic stroke. |
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