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| Action mechanism of apigenin 7-O-glucoside on the apoptosis of anti-TRAIL breast cancer cells |
| Hits 811 Download times 906 Received:August 10, 2023 |
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| DOI
10.11656/j.issn.1673-9043.2023.06.13 |
| Key Words
breast cancer;apigenin 7-O-glucoside;tumor necrosis factor-related apoptosis-inducing ligand;oxidative stress;apoptosis |
| Author Name | Affiliation | | ZHANG Naxian | Department of Breast Cancer, Nanyang Second People's Hospital, Nanyang 473000, China | | LIU Liu | Department of Breast Cancer, Nanyang Second People's Hospital, Nanyang 473000, China | | LI Gang | Department of Breast Cancer, Nanyang Second People's Hospital, Nanyang 473000, China | | LIU Linrui | Department of Breast Cancer, Nanyang Second People's Hospital, Nanyang 473000, China | | LI Yuanying | Department of Breast Cancer, Nanyang Second People's Hospital, Nanyang 473000, China |
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| Abstract
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| [Objective] To explore the effects of apigenin 7-O-glucoside (AGL) on the apoptosis of anti- tumor necrosis factor-related apoptosis inducing ligand (TRAIL) breast cancer cells.[Methods] The effects of AGL,TRAIL and AGL+TRAIL on activity of breast cancer cells,level of poly (ADP-ribose) polymerase-1 (PARP-1) and apoptosis were detected by CCK-8,Western-blot and flow cytometry. ROS level was detected by laser scanning confocal microscope. The level of malondialdehyde (MDA) and activity of superoxide dismutase (SOD) were detected by ELISA. The levels of PARP-1,p53 and p53up-regulated modulator of apoptosis (PUMA) were detected by Western-blot. MCF7 models of transplanted tumor were established to observe growth and apoptosis of transplanted tumor treated with AGL+TRAIL.[Results] With the treatment of TRAIL (50 ng/mL) +AGL (20,40 μmol/L),survival rate of MCF7 cells was decreased (P＜0.05),and Cleaved PARP-1 level was increased (P＜0.05). With the treatment of TRAIL (50 ng/mL) +AGL (20 μmol/L),apoptosis rate of MCF7 cells,levels of Cleaved-Cas-3 and Cleaved-Cas-9 were significantly increased (P＜0.05). With the treatment of AGL (10,20 μmol/L),number of ROS (+) cells and MDA level in MCF7 cells were significantly increased (P＜0.05),while SOD activity was significantly decreased (P＜0.05). The number of ROS (+) cells in MCF7 cells,related levels of p53,PUMA and Cleaved PARP-1 proteins with the treatment of TRAIL (50 ng/mL) +AGL (20 μmol/L) were significantly higher than those with the treatment of TRAIL (50 ng/mL) (P＜0.05),which were significantly lower with the treatment of TRAIL (50 ng/mL) +AGL (20 μmol/L) +NAC (4 mmol/L) than those with the treatment of TRAIL (50 ng/mL) +AGL (20 μmol/L) (P＜0.05). The mass and volume of transplanted tumors, positive rates of p53 and PUMA3 in AGL+TRAIL group were significantly lower than those in Control group (P＜0.05),while apoptosis rate was significantly higher than that in Control group (P＜0.05).[Conclusion] AGL combined with TRAIL can promote apoptosis of anti-TRAIL MCF7 cells,which may be related to enhancing oxidative stress damage and activating caspase cascade. |
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