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| Mechanism of Xiaoyan Decoction in treating gastric cancer based on network pharmacology |
| Hits 831 Download times 591 Received:August 02, 2023 |
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| DOI
10.11656/j.issn.1673-9043.2023.06.14 |
| Key Words
network pharmacology;Xiaoyan Decoction;gastric cancer;mechanism |
| Author Name | Affiliation | E-mail | | NIU Xiaofei | Dongying Vocational Institute, Dongying 257091, China | | | ZHANG Chang | General Hospital of Pingmei Shenma Group, Pingdingshan 467000, China | | | LI Xiaojiang | First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300381, China
National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin 300381, China | zxqlovelxj@126.com |
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| Abstract
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| [Objective] To explore the mechanism of Xiaoyan Decoction in the treatment of gastric cancer by network pharmacology.[Methods] TCMSP、TCMID and BATMAN-TCM database was used to screen the active ingredients and targets of Xiaoyan Decoction,and UniProt was used to obtain the corresponding gene names of protein targets;the gastric cancer disease targets were mined through DrugBank,GeneCardsand OMIM database,and the intersection genes with Xiaoyan Decoction target genes were screened;the drug compound intersection target network was drawn by using Cytoscape 3.7.2 software;the String number was used to calculate the sequence the PPI network was constructed according to the analysis platform,and the MCODE plug-in was used for cluster module analysis of PPI network;the Metascape database was used for KEGG enrichment analysis and GO enrichment analysis;the Kaplan-Meier Plotter database was used to explore the impact of key gene targets on OS of gastric cancer patients,and the survival curve was drawn.[Results] 41 active components,266 target genes,1 163 gastric cancer target genes and 116 cross genes were obtained,and 35 compounds were obtained after eliminating the active components without cross genes. TP53,JUN,AKT1,MAPK3,RELA,MAPK1,ESR1,MAPK14 and IL-6 are the key targets of Xiaoyan Decoction in the treatment of gastric cancer. The results of GO enrichment analysis showed that it was related to the signal pathway of apoptosis,response to inorganic substances,response to oxidative stress and response to chemical stress. The results of KEGG pathway enrichment analysis showed that it was mainly related to proteoglycan,PI3K-Akt signal pathway,IL-17 signal pathway,TNF signal pathway and platinum resistance in cancer. Survival analysis showed that the overall survival of patients with high expression of TP53,AKT1,MAPK3,RELA and ESR1 was significantly lower than that of low expression group,while the overall survival of patients with high expression of JUN,MAPK1 and MAPK14 was significantly higher than that of low expression group.[Conclusion] The research methods based on network pharmacology can help to find the key targets and signal pathways of Xiaoyan Decoction in the treatment of gastric cancer,and provide useful information and data support for further exploring the mechanism of Xiaoyan Decoction in the treatment of gastric cancer. |
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