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| Physapubescin induced human breast cancer MCF-7 cells apoptosis by inhibiting STAT3 |
| Hits 480 Download times 371 Received:October 09, 2023 |
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| DOI
10.11656/j.issn.1673-9043.2024.01.02 |
| Key Words
human breast cancer MCF-7 cell;Physapubescin;apoptosis;TAT3 |
| Author Name | Affiliation | E-mail | | HAN Hongye | School of Intergrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
School of Medical Technology, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | YU Yaqin | School of Intergrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Department of Oncology, Wuhan Huangpi District People's Hospital, Wuhan 430300, China | | | ZHANG Qiang | School of Medical Technology, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | SUN Yujie | School of Medical Technology, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | KANG Ning | School of Medical Technology, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | kangndd@163.com |
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| Abstract
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| [Objective] This study determined the role of signal transducer and activator of transcription 3(STAT3) in Physapubescin(PPB) induced apoptosis in the human breast cancer MCF-7 cells. [Methods] The fluorescent stain technology was used to analyze that PPB induced MCF-7 cells apoptosis. The potential mechanisms of PPB against breast cancer were predicted by bioinformatics analysis. The MTT assay was used to investigate the effects of STAT3 inhibitor S3I-201 and STAT3 small interfering RNA(siRNA) on the inhibition of MCF-7 cell growth by PPB. The effects of PPB alone or pretreatment with STAT3 siRNA on the expression of STAT3, B-cell lymphoma-2(Bcl-2), Bcl-2 Associated X protein(Bax), Caspase8, Caspase9, Cytochrome c and poly polymerase(PARP) proteins in MCF-7 cells were detected by Western Blot. [Results] The apoptotic morphology of MCF-7 cells was distinctly characterized by the effect of PPB, and the apoptosis ratio was significantly increased.The bioinformatics analysis showed that STAT3, a common target of PPB and breast cancer disease, was highly expressed in breast cancer tissues, and single-gene GSEA results suggested that high STAT3 expression was negatively correlated with the apoptotic signaling pathway. The results of Western Blot showed that PPB could inhibit STAT3 phosphorylation. The S3I-201 inhibitor or STAT3 siRNA could further promote PPB to inhibit MCF-7 cell growth. In addition, knockdown of STAT3 further increased the up-regulation of PPB on the pro-apoptotic proteins Bax, Cytochrome c, Cleaved-Caspase8, Cleaved-Caspase9, and Cleaved-PARP, meanwhile the inhibitory effect of PPB on the expression of anti-apoptotic protein Bcl-2 was enhanced by treatment with STAT3 siRNA. [Conclusion] PPB induced human breast cancer MCF-7 cells apoptosis by inhibiting STAT3. |
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