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| Analysis on the determination and quantity transfer of multi index components in Qianghuo Shengshi Decoction |
| Hits 543 Download times 390 Received:August 30, 2023 |
| View Full Text View/Add Comment Download reader |
| DOI
10.11656/j.issn.1673-9043.2024.01.03 |
| Key Words
classical prescription;Qianghuo Shengshi Decoction;substance benchmark;quantity transfer |
| Author Name | Affiliation | E-mail | | WANG Min | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
State Key Laboratory of Multi-fractions Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | XUE Zhifeng | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
State Key Laboratory of Multi-fractions Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | SUN Xinru | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
State Key Laboratory of Multi-fractions Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LI Dandan | Harbin Zhenbao Pharmaceutical Co., Ltd., Harbin 150040, China | | | WANG Zhijuan | Harbin Zhenbao Pharmaceutical Co., Ltd., Harbin 150040, China | | | HUI Yuyu | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
State Key Laboratory of Multi-fractions Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LIU Zhidong | Engineering Research Center of Modern Chinese Medicine Discovery and Preparation, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
State Key Laboratory of Multi-fractions Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | liuzhidong@tjutcm.edu.cn |
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| Abstract
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| [Objective] Establish the High Performance Liquid Chromatography(HPLC) content determination method of multi-index components in Qianghuo Shengshi Decoction(QSD) substance benchmarks, to analysis the quantity transfer of multi-index components. [Methods] Preparing 15 batches of substance benchmarks freeze-drying powder(lyophilized powder) samples of QSD. The specific components, including Notopterol, Columbianadin, 5-O-methylvisammioside, Vitexicarpin, Isoimperatorin, and Ammonium Glycyrrhizinate were used as indexes to establish the HPLC method for quantitative evaluation, and define the contents and the transfer rate range of multi-index components and the paste-forming rate range to analysis on the quantity transfer of multi index components in decoction pieces to substance benchmarks. [Results] The contents and transfer rates of the 15 batches of substance benchmarks were 1.09~1.70 mg/g and 10.17%~32.62% of Notopterol;Columbianadin were 0.06~0.20 mg/g and 7.30%~10.85%;5-O-methylvisammioside were 1.27~2.81 mg/g and 66.13%~92.13%;Vitexicarpin were 0.09~0.59 mg/g and 5.24%~13.37%;Isoimperatorin were 0.03~0.28 mg/g and 0.94%~1.92%;Ammonium Glycyrrhizinate were 12.74~33.78 mg/g and 48.96%~62.56%, respectively. The paste-forming rate ranged from 30.09% to 34.35%. [Conclusion] The content determination method of standard multi-index components of classical famous prescription QSD was preliminarily established by HPLC, which provided a basis for the quality control of the follow-up research and the development of related preparations of QSD. |
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