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| Analysis of chemical constituents in Wendan Decoction based on UPLC-Q-Exactive Orbitrap-MS |
| Hits 407 Download times 647 Received:September 18, 2023 |
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| DOI
10.11656/j.issn.1673-9043.2024.02.05 |
| Key Words
Wendan Decoction;UPLC-Q-Exactive Orbitrap-MS;component analysis;ancient classic Chinese medicine formula |
| Author Name | Affiliation | E-mail | | ZHANG Kuibin | State Key Laboratory of Component Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | XING Bin | State Key Laboratory of Component Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | SONG Wen | Tianjin Hongrentang Pharmaceutical Co., Ltd, Tianjin 300122, China | | | WANG Renxing | State Key Laboratory of Component Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | ZHANG Qingqing | State Key Laboratory of Component Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | | | LIU Zhidong | State Key Laboratory of Component Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | liuzhidong@tjutcm.edu.cn | | PI Jiaxin | State Key Laboratory of Component Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China | pijiaxin@tjutcm.edu.cn |
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| Abstract
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| [Objective] To rapidly identify the chemical components of Wendan Decoction by UPLC-Q-Exactive Orbitrap-MS. [Method] Waters Acquity BEH C18(2.1×100 mm,1.7 m) was used for gradient elution with 0.1% formic acid aqueous solution(A)-Acetonitrile(B) as mobile phase in positive and negative ion modes. The flow rate was 0.3 mL/min,the column temperature was 30℃,and the injection volume was 2 L. Adopting heated electrospray ion source(HESI),scan mode is Full MS/dd-MS2 and scan range m/z 100~1500. [Results] The compound was identified by comparing with the accurate relative molecular mass,multi-stage mass spectrometry fragment information,combining with literature reports and mass database information. A total of 121 ingredients were identified from Wendan Decoction,including 70 flavonoids,18 coumarins,9 saponins,11 nitrogen-containing ingredients,3 phenylpropanoids and 10 other components. Among them,15 components were confirmed after comparing with the reference substance. [Conclusion] UPLC-Q-Exactive Orbitrap-MS technology was used to systematically clarify the basic ingredients of Wendan Decoction,which can lay a foundation for the better research of the pharmacodynamic material basis and the reasonable formulation of quality standards. |
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