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| Investigating the effects of Wenjing Decoction combined with Hupo Powder on cold-congealed blood stasis in endometriosis rats,using the NF-κB pathway |
| Hits 744 Download times 467 Received:December 01, 2023 |
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| DOI
10.11656/j.issn.1673-9043.2024.04.05 |
| Key Words
endometriosis;Wenjing Decoction combined with Hupo Powder;NF-κB signalling pathway;cold-congealed blood stasis |
| Author Name | Affiliation | E-mail | | DU Zifei | Guangzhou Medical University Hospital of Traditional Chinese Medicine, Guangzhou 510130, China | | | SHI Xinrui | Guangzhou Medical University Hospital of Traditional Chinese Medicine, Guangzhou 510130, China | | | YUAN Xiaoyi | Guangzhou Medical University Hospital of Traditional Chinese Medicine, Guangzhou 510130, China | | | WANG Bingyu | Guangzhou Medical University Hospital of Traditional Chinese Medicine, Guangzhou 510130, China | | | WANG Yong | Guangzhou Medical University Hospital of Traditional Chinese Medicine, Guangzhou 510130, China | touger20@163.com |
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| Abstract
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| [Objective] To explore the effects of Wenjing Decoction combined withHupoPowderon cold-congealed blood stasis endometriosis(EMs) rats based on the nuclear transcription factor-κB(NF-κB) pathway and related proteins. [Methods] Sixty SPF-grade female SD rats were randomly divided into sham-operated group,model group,gestrinone categories(GC),and low,medium,and high dose groups(9.69,19.38 and 38.75 g/kg) of Wenjing Decoction combined with Hupo Powder group,with 10 rats in each group. Except for the sham-operated group,the rat model of EMs with cold-congealed blood stasis was established by using ice-water bath combined with epinephrine hydrochloride and autologous endothelial transplantation in all groups of rats. Sham-operation group with only open abdomen without endometrial transplantation. The sham-operated and model groups were given pure water. The progesterone group was given progesterone(0.25 mg/kg) by gavage twice a week(every Monday and Thursday) dissolved in pure water for four weeks. The group of Wenjing Decoction with Hupo Powder was given low dose of Wenjing Decoction with Hupo Powder(9.69 g/kg),medium dose of Wenjing Decoction with Hupo Powder(19.38 g/kg),and low dose of Wenjing Decoction with Hupo Powder(38.75 g/kg) by gastric gavage for four consecutive weeks,respectively. Serum tumor necrosis factor-α(TNF-α) and interleukin 1β(IL-1β) levels were measured using enzyme-linked immunosorbent assay(ELISA) in each group of rats and the blood rheology(plasma viscosity,erythrocyte aggregation index,erythrocyte rigidity index,erythrocyte deformation index,erythrocyte electrophoresis time),coagulation[prothrombin time(PT),prothrombin time international normalized ratio(PT-INR),partial thromboplastin time(TT),fibrinogen(FIB)] was tested in each group of rats. Detection of NF-κB p65,IkBα,and IKKβ protein expression in endometrial tissues of rats in each group using protein immunoblotting. [Results] Compared with the sham-operated group,the serum TNF-α and IL-1β levels were significantly higher in the model group(P<0.05 or P<0.01),and all the indices of blood rheology were significantly higher(P<0.05 or P<0.01). For coagulation,TT was significantly lower(P<0.05) and FIB were significantly higher(P<0.01). The results of protein expression blotting assay showed that NF-κB p65 and IKKβ were significantly elevated(P<0.01),and the expression of IkBα was significantly reduced(P<0.05). Compared with the model group,the expression of serum TNF-α and IL-1β was significantly reduced in the western medicine and part of traditional Chinese medicine(TCM) dose groups(P<0.05 or P<0.01),as well as all the indexes of blood rheology(P<0.05 or P<0.01). For coagulation,TT were significantly higher(P<0.05 or P<0.01) and FIB were significantly lower(P<0.05 or P<0.01). The results of protein expression blotting assay showed that the expression of NF-κB p65 were decreased(P<0.05 or P<0.01),the expression of IKKβ in the western medicine group and part of TCM dose groups were significantly decreased(P<0.05 or P<0.01),the expression of IkBα in the western medicine and part of TCM dose groups were increased(P<0.05 or P<0.01). [Conclusion] Wenjing Decoction combined with Hupo Powder may shrink the ectopic foci and regulate the development of endometriosis by blocking or inhibiting the activation of the NF-κB signalling pathway and inhibiting the proliferation of ectopic tissues,and it can improve the symptoms of cold agglutination and blood stasis,with a therapeutic efficacy superior to that of pregnenolone. |
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